in order to improve simultaneous detection and identification of helicobacter genus in general and helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex pcr. in this study, two pairs of primers: hcom1 and hcom2 specific for helicobacter genus, hicd1 and hicd2 specific for helicobacter pylori species were used. to determine the sensitivity of...

the opportunistic pathogen c.albicans is able to cause disseminated infections in immunocompromised patients. microbiological methods for the diagnosis of invasive candidiasis have many problems including low sensitivity, requirement to invasive clinical sampling such as biopsies or multiple blood cultures and need to expertise laboratory stuff. since pcr has proven to be a powerful tool in the...

background: in most cases, prostatitis can be caused by a bacterial agent such as ureaplasma urealyticum. considering to the cumbersome of the culture method for the detection of ureaplasma species in clinical samples such as prostate; pcr method that is faster and more appropriate than the cultivation methods, can be utilized for the detection of u. urealyticum and u. parvum. pcr-rflp method c...

Satureja genus presents 12 aromatic species in Iran, eight of which are endemic. Because of the several usages of Satureja species or their oils in different industries, we were interested to study the oil content and composition of Iranian Satureja species. In this research, the essential oil content and composition of two endemic species (S. edmondi and S. isophylla) were investigated. Essent...

background: the free-living amoebae acanthamoeba spp. have been recognized as etiologic agents of amoebic encephalitis, keratitis, otitis, lung lesions and other skin infections mainly in immuno-compromised individuals. the purpose of this study is to detect the presence of acanthamoeba in swimming pools in egypt using a polymerase chain reaction (pcr) method. methods: water samples were collec...

background: salmonella enterica serovar typhi, as causative agent of typhoid fever, is one of the most important endemic pathogens. non-typhoidal salmonella serovars, including typhimurium , infantis , and enteritidis are amongst the most prevalent serotypes worldwide and in developing areas such as iran. the aim of this study was to apply a uniplex pcr for rapid detection of salmonella spp., a...