We evaluated a new method for measurement of acetaminophen. From acetaminophen, acetate and p-aminophenol are enzymically produced, and the latter product is determined colorimetrically by reaction with o-cresol and ammoniacal copper sulfate. The method is rapid and precise, and results compare well with those by spectrophotometric and liquid-chromatographic techniques. Major metabolites of ace...

The measurement of plasma hemoglobin is utilized in the evaluation of various hemolytic disorders. The purpose of this report is to illustrate the advantages of a direct spectrophotometric scanning technique for determination of of plasma hemoglobin. This method does not require the use of carcinogenic reagents. The scanning method demonstrates linearity, precision, and sensitivity suitable for...

In this Laboratory Exercise, we will determine the amount of active ingredient in a commercial face wash; Sodium Salicylate. We will accomplish this by conducting a spectrophotometric analysis of the preparation at the wavelength of maximal absorbance for this compound when complexed with Fe 3+ . The resulting absorbance measurement will be correlated against calibration standards, using Beer’s...

A comparison of the distribution of CO oxidation activity between soluble and particulate protein fractions obtained after disruption of CO-grown Rhodopseudomonas gelatinosa 1 by French pressure cell breakage and osmotic lysis of spheroplasts suggested that, in situ, the enzyme complex was associated with the cell membrane. An improved, strictly anaerobic method is given for spectrophotometric ...

We describe an improved determination of phenylpyruvic acid (PPA) in blood plasma by ultraviolet spectrophotometric measurement of an enolborate complex. Sensitivity and recovery are well suited for accurate analy. sis in the range of 1.25 to 25 mg/liter. Sera from 10 fasting phenylketonuric subjects averaged 15.3 mg/liter (range, 4.8 to 25.0 mg/liter). A pronounced diurnal rhythm in plasma PPA...

Novel, simple, sensitive, rapid, accurate and economical spectrophotometric methods have been developed for simultaneous estimation of Nebivolol Hydrochloride and Hydrochlorothiazide. These methods involve solving of simultaneous equations based on measurement of absorbances at two wavelengths 290 nm and 317 nm. Both the drugs obey the Beer’s law in the concentration ranges employed for these m...

In this Laboratory Exercise, we will determine the amount of active ingredient in a commercial face wash; Sodium Salicylate. We will accomplish this by conducting a spectrophotometric analysis of the preparation at the wavelength of maximal absorbance for this compound when complexed with Fe 3+ . The resulting absorbance measurement will be correlated against calibration standards, using Beer’s...

The Carba NP test was evaluated against a panel of 61 carbapenemase-producing bacterial species (15 producing class A carbapenemases, 15 producing class D carbapenemases, and 31 producing metallo-β-lactamases) and against 111 isolates with non-wild-type carbapenem susceptibility but not producing carbapenemase. Carbapenemase production was verified by PCR and UV-spectrophotometric measurement o...