AIMS To determine sperm nuclear DNA integrity and to investigate the relation between fenvalerate (FE) exposure and spermatozoa DNA damage. METHODS Sperm DNA fragmentation was detected by a modified alkaline single cell gel electrophoresis (Comet) assay and a terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL) assay. The olive tail moment (OTM) and percentage tail D...

OBJECTIVE To test whether sperm with abnormal head morphology are more likely to undergo DNA damage and/or chromatin modification during the process of freeze-thawing. DESIGN In this prospective study, the semen samples from forty-four men attending the infertility clinic were included. Samples were divided into aliquots to allow direct comparison of fresh and frozen spermatozoa from the same...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Agarwal et al. in their most recent paper (1) propose a clinical guideline on the use of sperm DNA damage testing in infertility treatment. This guideline is extremely relevant for fertility specialists and further insight into the topic is in high demand as the debate regarding the role of DNA damage testing is still...

OBJECTIVE To compare the sperm chromatin dispersion (SCD) test and the terminal uridine nick-end labeling (TUNEL) assay for assessment of sperm DNA damage. DESIGN Prospective comparative experimental study. SETTING Andrology laboratory. PATIENT(S) Twenty subfertile men with unexplained infertility. INTERVENTION(S) Sperm DNA damage was determined in the same semen samples using the TUNEL...

STUDY QUESTION Is there an association between sperm DNA damage, measured by three different assays, sperm nuclear protein content and clinical outcomes in assisted reproduction treatment (ART)? SUMMARY ANSWER Sperm DNA damage measured by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) and the Comet assay were significantly associated with ART outcomes in our sin...

INTRODUCTION Although the pathophysiology of the testicular damage associated with varicocele remains unclear, sperm DNA damage has been identified as a potential explanation for this cause of male infertility. The current study was designed to determine the extent of sperm nuclear DNA damage in patients with varicocele, and to examine its relationship with parameters of seminal motility. MAT...

background: currently, relation between reactive oxygen species (ros) ros concentration and semen quality was indicated. saffron has traditionally been not only considered as a food additive but also as a medicinal herb, which has a good antioxidant properties. objective: the aim of this study was to evaluate the protection potency of saffron and vitamin e on sperm chromatin integrity. material...

varicocele is one of the most common causes of primary male infertility. pilea microphylla (pm) is being used as folk medicine. this study was aimed to investigate the effects of pm in a rat model of varicocele. a total of 30 male wistar rats were divided into control, sham, varicocele, accessory varicocele and pm-treated groups. after 10 weeks of varicocele induction, sperm parameters and chro...

UNLABELLED The objectives of this cross-sectional observational study were: (i) to detect DNA damage and plasma membrane translocation of phosphatidylserine in purified sperm populations of high and low motility, and (ii) to analyse their relationship with the endogenous generation of reactive oxygen species. Ejaculates from infertile men were examined following gradient centrifugation. The mai...

A wide variety of techniques for the preparation of sperm are currently available, of which the most commonly employed are density-gradient centrifugation (DGC) and swim-up (SUP). To date, these methods appear to be effective in selecting functional sperm for assisted reproduction techniques (ART), but they may have negative effects on sperm DNA. In this study, the ability of these semen proces...