background new research proposes the pluripotency of spermatogonial cells obtained from testis. these spermatogonia-derived stem cells are called embryonic stem-like cells that express embryonic stem cell markers and differentiate to the three germ layers. objectives the aim of the present study was to generate embryonic stem-like cells from neonatal mouse testis. materials and methods the test...

Stem cells represent a unique population of cells with self-renewal capacity. Although they are important therapeutic targets, the genetic manipulation of tissue-specific stem cells has been limited, which complicates the study and practical application of these cells. Here, we demonstrate successful gene trapping and homologous recombination in spermatogonial stem cells. Cultured spermatogonia...

BACKGROUND Spermatogonial stem cell (SSC) is a self-renewing population of male adult stem cell. SSCs have a differentiation potential which are similar to embryonic stem cells. These Embryonic stem like (ES-like) cells can be a potential source for pluripotent cells for stem cell-based therapy. OBJECTIVE This study presents an economical and simple co-culture system for pluripotent stem cell...

BACKGROUND The development of a spermatogonial transplantation technique has provided new possibilities for the treatment of male infertility. Previous studies have shown that spermatogonial stem cells could reinitiate spermatogenesis after cryopreservation and reintroduction into the seminiferous tubules of infertile recipient males, and this raised the possibility of banking frozen stem cells...

Spermatogonial stem cells (SSCs) are the only stem cells in adults that can transfer genetic information to the future generations. Considering the fact that a single SSC gives rise to a vast number of spermatozoa, genetic manipulation of these cells is a potential novel technology with feasible application to various animal species. The aim of this study was to evaluate enhanced green fluoresc...

background herbal extracts have recently received the greatest attention in the path of finding naturally occurring chemicals with antibacterial and therapeutic value; however, each type of herbal remedy may have its own side effects. objectives the aim of the current experiment was to study the antibacterial effect of myrtle, parsley, mint, henna and chamomile extracts on escherichia coli and ...

Spermatogonia are the male germ stem cells that continuously produce sperm for the next generation. Spermatogenesis is a complicated process that proceeds through mitotic phase of stem cell renewal and differentiation, meiotic phase, and postmeiotic phase of spermiogenesis. Full recapitulation of spermatogenesis in vitro has been impossible, as generation of normal spermatogonial stem cell line...

spermatogonial stem cell (ssc) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. the therapeutic use of embryonic stem cells (escs) is restricted due to severe ethical and immunological concerns. therefore, we need a new pluripotent cell type. despite well-known role of germ cells in the gametogenesis, some facts apparently show th...