Changes in the level of a testis-specific hsp70 gene-related transcript (hst70 RNA) and its cellular localization during the cycle of rat seminiferous epithelium have been investigated. Segments of seminiferous tubules at defined stages of the cycle were isolated in living condition by transillumination-assisted microdissection and the exact stages identified by phase-contrast microscopy of liv...

The autosomal gene DAZL is a member of a family of genes (DAZL, DAZ, BOULE), all of which contain a consensus RNA binding domain and are expressed in germ cells. Adult male and female mice null for Dazl lack gametes. In order to define more precisely the developmental stages in germ cells that require Dazl expression, the patterns of germ cell loss in immature male and female wild-type (+/+, WT...

Progression through the prophase of the first meiotic division can be obtained in culture by treatment of mouse spermatocytes with the serine/threonine phosphatase inhibitor okadaic acid. Chromosome condensation during this G2/M transition involves the activation of the MAPK pathway, which causes the activation of Nek2 and the phosphorylation of the chromatin architectural protein Hmga2. In an ...

Predication facilitates high-bandwidth fetch and large static scheduling regions, but has typically been too complex to implement comprehensively in out-of-order microarchitectures. This paper describes dataflow predication, which provides per-instruction predication in a dataflow ISA, low predication computation overheads similar to VLIW ISAs, and low complexity out-of-order issue. A twobit fi...

Autoradiographs of adult mice killed at various times after injection of tritiated thymidine show significant numbers of labelled nuclei in organs in which mitoses are either very rare or completely absent. The proportion of labelled cells that divide was estimated from the decline in the number of grains per nucleus, the number of pairs of labelled cells in sheets of epithelium in squashes, th...

T HE title of this article was chosen for two reasons. First, it is the title of a remarkable article published 50 years ago by BORIS EPHRUSSI and EILEEN SUTTON aimed at explaining a widely studied but enigmatic phenomenon (EPHRUSSI and SUTTON 1944). But in addition, by highlighting ideas presented in that article, I will be reconsidering the mechanism of position effect in light of recent work...

An investigation of the structure of meiotic chromosomes from primary spermatocytes of two salamanders, Plethodon cinereus and Desmognathus fusca, has been made using correlated light and electron microscopy. Feulgen squashes were compared with stained sections and these related to adjacent thin sections in the electron microscope. A transition from the familiar cytological preparation to the e...

BACKGROUND AND AIMS Polyploidy is viewed as an important mechanism of sympatric speciation, but only a few studies have documented patterns of distribution and ecology of different cytotypes in their contact zone. Aster amellus agg. (Asteraceae) is one of the species with documented multiple ploidy levels. The aim of this study was to determine spatial distribution and ecology of two cytotypes,...

BACKGROUND Documenting the spectrum of ecosystem management, the roles of forestry and agricultural biodiversity, TEK, and human culture for food sovereignty, are all priority challenges for contemporary science and society. Ethnoagroforestry is a research approach that provides a theoretical framework integrating socio-ecological disciplines and TEK. We analyze in this study general types of A...

TUDIES of the types of aberrations induced in chromosomes by irradiation S during the meiotic prophase have been infrequent. The complications introduced by synapsis and crossing over make the analysis much more difficult than is the case for mitotic divisions on which most of the work has been concentrated. However, in the assessment of radiation damage to genetic material, the differential se...