نتایج جستجو برای: ssc coculture

تعداد نتایج: 13494  

2014
Monique R. Arts Murray Baron Nidaa Chokr Marvin J. Fritzler Marc J. Servant

OBJECTIVE It has been suggested that autoantibodies in systemic sclerosis (SSc) may induce the differentiation of cultured fibroblasts into myofibroblasts through platelet-derived growth factor receptor (PDGFR) activation. The present study aims to characterize the effects of SSc IgG on vascular smooth muscle cells (VSMCs) and to determine if stimulatory autoantibodies directed to the PDGFR can...

2016
Lesley-Anne Bissell Giuseppina Abignano Paul Emery Francesco Del Galdo Maya H. Buch

BACKGROUND To report the predictive value of nail-fold capillaroscopy (NFC) patterns of vasculopathy for systemic sclerosis (Scleroderma; SSc) in an unselected cohort of patients with Raynaud's phenomenon (RP). METHODS Patients referred to a tertiary SSc clinic with RP were evaluated by light/video-NFC. Clinical diagnosis, details and serology were recorded. Primary RP was defined as RP with ...

Journal: :Annals of the rheumatic diseases 2012
Eugénie Koumakis Julien Wipff Philippe Dieudé Barbara Ruiz Matthieu Bouaziz Lucile Revillod Mickaël Guedj Jörg H W Distler Marco Matucci-Cerinic Marc Humbert Gabriella Riemekasten Paolo Airo Inga Melchers Eric Hachulla Daniele Cusi H-Erich Wichmann Nicolas Hunzelmann Kiet Tiev Paola Caramaschi Elisabeth Diot Otylia Kowal-Bielecka Giovanna Cuomo Ulrich Walker László Czirják Nemanja Damjanov Sara Lupoli Costanza Conti Martina Müller-Nurasyid Ulf Müller-Ladner Valeria Riccieri Jean-Luc Cracowski Franco Cozzi Vasiliki Kalliopi Bournia P Vlachoyiannopoulos Gilles Chiocchia Catherine Boileau Yannick Allanore

INTRODUCTION Systemic sclerosis (SSc)-related pulmonary arterial hypertension (PAH) has emerged as a major mortality prognostic factor. Mutations of transforming growth factor beta (TGFβ) receptor genes strongly contribute to idiopathic and familial PAH. OBJECTIVE To explore the genetic bases of SSc-PAH, we combined direct sequencing and genotyping of candidate genes encoding TGFβ receptor ...

2017
Stefanie Friedrich Susanne Lüders Stephanie Gabriele Werner Anne-Marie Glimm Gerd-Rüdiger Burmester Gabriela Riemekasten Marina Backhaus Sarah Ohrndorf

BACKGROUND Utilising fluorescence optical imaging (FOI), the distribution of an intravenously applied colouring agent indocyanine green (ICG) can be analysed with the potential to identify malperfusion by little to no tissue enhancement. Systemic sclerosis (SSc) is characterised by the presence of digital ulcers reflecting progressive vasculopathy. The objective was to investigate the potential...

Journal: :Acta clinica Belgica 2016
Els H Vandecasteele Michel De Pauw Guy Brusselle Saskia Decuman Yves Piette Filip De Keyser V Smith

Systemic sclerosis (SSc) is an autoimmune connective tissue disease characterized by vasculopathy and progressive fibrosis of the skin and visceral organs (gastrointestinal tract, heart, kidneys and lungs). Although the prevalence is low, SSc is a disease with high morbidity and mortality. Since pulmonary arterial hypertension (PAH) associated with SSc (SSc-PAH) and clinically evident cardiac i...

Journal: :Jornal brasileiro de pneumologia : publicacao oficial da Sociedade Brasileira de Pneumologia e Tisilogia 2009
Edwin Roger Parra Leandro Hideki Otani Erika Franco de Carvalho Alexandre Ab'Saber Vera Luiza Capelozzi

OBJECTIVE The aim of this study was to examine the parenchymal and extracellular matrix remodeling process in two histologic patterns-nonspecific interstitial pneumonia (NSIP) and usual interstitial pneumonia (UIP)-in cases of idiopathic and sclerosis/systemic sclerosis (SSc)-associated interstitial pneumonia. METHODS We examined 15 cases of idiopathic NSIP, 10 cases of idiopathic UIP, 5 case...

Journal: :international journal of aquatic biology 0
paramita das department of zoology, the university of burdwan koushik ghosh department of zoology, the university of burdwan

sesame oil cake (ssc) was bio-processed through solid state fermentation (ssf) under optimized conditions by a phytase-producing fish gut bacterium, bacillus subtilis subsp. subtilis (jx292128). ssf significantly reduced anti-nutritional factors (e.g., phytic acid, tannins and trypsin inhibitor) and crude fibre, while enhanced free amino acids, fatty acids and different minerals. phytase product...

Journal: :Animal reproduction science 2000
J F Hasler

The present study was designed to test the efficacy, as assessed by blastocyst production, of culturing slaughterhouse-derived bovine oocytes in Ménézo's B2 (B2) medium with or without serum and with or without buffalo rat cell (BRL) coculture. In addition, OPU-derived oocytes were cultured in B2-BRL coculture with or without serum for the first 72 h or TCM 199-BRL coculture with serum and the ...

Journal: :Clinical and diagnostic laboratory immunology 1997
U K Sharma H F Song F F Willingham J Hannig C Flexner H Farzadegan C Nicolau D H Schwartz

Standard isolation of human immunodeficiency virus type 1 (HIV-1) from peripheral blood mononuclear cells (PBMC) requires 5 to 20 ml of blood, and the centrifugal separation of PBMC is expensive and time-consuming. Whole-blood coculture techniques use small sample volumes, do not require centrifugation, and allow measurement of the total viral burden in peripheral circulation. We compared the r...

2016
Hui Huang Rui E. Feng Shan Li Kai Xu Ya Lan Bi Zuo Jun Xu

Systemic sclerosis (SSc)-associated interstitial lung disease (SSc-ILD) has become the leading SSc-related cause of death. Although various types of immunosuppressive therapy have been attempted for patients with SSc-ILD, no curative or effective treatment strategies for SSc-ILD have been developed. Therefore, management of patients with SSc-ILD remains a challenge. Here, we report a Chinese, f...

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