we searched for novel chinese hamster ovary (cho) cell mutants defective in peroxisome biogenesis by an improved method using peroxisome targeting sequence (pts) of pex3p (amino acid residues 1–40)-fused enhanced green fluorescent protein (egfp). from mutagenized tkaeg3(1–40) cells, the wild-type cho-k1 stably expressing rat pex2p and of rat pex3p(1–40)-egfp, numerous cell colonies resistant to...

proteinase 3(pr3) is a human polymorphonuclear leukocyte serine proteinase and is the main target antigen for antineutrophil cytoplasmic antibodies (anca) found in wegener's granulomatosis (wg). we developed a stable expression system for conformationally intact recombinant pr3 (rpr3) in chinese hamster ovary cells (cho-cells). the part of pr3 cdna that encoded the active form of pr3 was s...

The rabies virus glycoprotein (G protein) has several important functions and is a major antigenic stimulus of the host immune system following rabies virus infection or vaccination. We developed a model system for studying the role of N-linked glycosylation in the intracellular transport and antigenicity of this molecule. The full-length cDNA of the G protein of the ERA strain of rabies virus ...

We have examined the role of cell surface glycosaminoglycans in cell division: adhesion and proliferation of Chinese hamster ovary (CHO) cells. We used both wild-type (CHO-K1) cells and a mutant (CHO-745) which is deficient in the synthesis of proteoglycans due to lack of activity of xylosyl transferase. Using different amounts of wild-type and mutant cells, little adhesion was observed in the ...

BACKGROUND During development of recombinant monoclonal antibodies in Chinese hamster ovary (CHO) cells, C-terminal amidated species are observed. C-terminal amidation is catalysed by peptidylglycine α-amidating monooxygenase (PAM), an enzyme known to be expressed in CHO cells. The significant variations between clones during clone selection, and the relatively high content of amidated species ...

The phosphorylation of H1 histone subtypes was studied in 3 Chinese hamster cell lines (CHO, V79, and CHW). Chromatographic resolution of H1 subtypes showed that all 3 cell lines contained 1 homologous (coeluting) H1 subtype (CHO-1, V79-1, and CHW-1) while V79 and CHW cells contained 2 additional H1 subtypes not found in CHO cells (V79-2,3 and CHW-2,3). N-Bromosuccinimide cleavage of 32P-labele...

objective: the study of physiological changes in recombinant cell lines provides useful information to improve production performance. in this study, we investigate the effects of an anti-cd33 chimeric igg4 expression on sp2.0 cell growth. methods: variable region genes of light and heavy chains of monoclonal antibody produced by m195 were cloned in pfuse-clig-hk and pfuse-chig-hg4 expression ...

The use of Chinese Hamster Ovary (CHO) cells for transient gene expression is gaining importance steadily, as it has been shown that both quality and quantity of proteins derived from CHO cells differs from and can even be superior to material derived from Human Embryonic Kidney (HEK293) cells [1-4]. In order to augment yields HEK cell lines have been genetically modified, by e.g. stable integr...