The study has strived to compare the leucocyte-esterase stripe activity on sputum samples with gram-stained direct smear microscopy. This is for the demonstration of white blood cells (WBCs) and so to develop a simple method for selecting suitable sputum samples for cultural examination and laboratory diagnosis. . A total of 17(34.0%) out of 50 samples studied were shown to be unsuitable fo...

BACKGROUND The microscopical diagnosis of male urethritis was recently questioned by Rietmeijer and Mettenbrink, lowering the diagnostic criteria of the diagnosis to ≥2 polymorphonuclear leucocytes (PMNL) per high power field (HPF), and adopted by Centers for Disease Control and Prevention in their 2015 STD Treatment Guidelines. The European Non-Gonococcal Urethritis Guideline advocates a limit...

In the last years, digital image processing and analysis are used for computer assisted evaluation of semen quality with therapeutic goals or to estimate its fertility by means of spermatozoid motility and morphology. Sperm morphology is assessed routinely as part of standard laboratory analysis in the diagnosis of human male infertility. Nowadays assessments of sperm morphology are mostly done...

Tubercle bacilli, which survived heat fixation, were detected with a slide culture technique which allowed the entire smear to be examined. Both conventional flame fixation and the use of a controlled hot-plate failed to render tuberculous sputum smears safe for further handling. Smears which were stained with the phenol-auramine method failed to yield growth on culture. If delay between prepa...

introduction: the penetration depth of irrigating solutions in dentinal tubules is limited; consequently, bacteria can remain inside dentinal tubules after the cleaning and shaping of the root canal system. therefore, new irrigation systems are required to increase the penetration depth of irrigating solutions in dentinal tubules. methods: a comparative study regarding the penetration depth of ...

seventy-five years. Various opinions have been expressed, based on examination of fixed and stained tissue sections,’ 7-10 smear preparations1’ 6 and tissue culture.” Recent discussions of the conflicting opinions can be found in review articles by Symmers’#{176} and Hoster and After exploring the possibilities of phase contrast microscopy in cytologic studies of normal and diseased lymphoid ti...

The use of a dipstick to detect leucocyte esteraseuria (Chemstrip L) was compared with the visualisation of leucocytes in a Gram stained smear for the detection of pyuria. The sensitivity and specificity of the two systems using the predictive value theory were similar. The use of a dual screening procedure (automation plus dipstick) allowed reliable 4 h screening of urine specimens for the det...

11 Confirmative diagnosis of Visceral Leishmaniasis (VL) is still being a challenge at the primary 12 health care facilities in most of the rural endemic areas in the Indian sub-continent. Conventional 13 methods for parasitological confirmation are risky and require skilled personnel hence, 14 unreachable to the poor people in the endemic region. Buffy coat smear microscopy, as a 15 minimally ...

A simple method for treating bovine blood samples for direct detection of T. annulata in carriers, after polymerase chain reaction (PCR) amplification of small subunit ribosomal RNA (SSU rRNA) gene is described. The threshold of detection of the PCR-assay was an erythrocytic parasitaemia of 0.00008% corresponding to 16 infected bovine erythrocytes. In 50 known carriers, 42 were positive in PCR,...

We evaluated the reliability of Staphaurex (Wellcome Diagnostics, Dartford, England) for the direct identification of staphylococci from blood culture broth with evidence of positivity and a suggestive Gram-stained smear. Our evaluation indicates that this application is of limited sensitivity, thus reducing the value of a negative test. However, since the test is highly specific, a positive te...