Negative staining technique of Heine is an easy, inexpensive, and rapid way of screening for coccidian parasites of the intestinal tract. But its use as a routine technique for screening of Cryptosporidium, Cyclospora, and Cystoisospora is restricted due to its sensitivity being lower than the gold standard method of modified Ziehl-Neelsen staining. This paper emphasises the modification of ori...

Remazolbrilliant Blue covalently linked to amylose (Amylose Azure) has been used for the determination of ex-amylase (Rinderknecht, Wilding, and Haverback, 1967). It forms an insoluble suspension in aqueous media, but liberates a soluble highly coloured blue dye as a result of amylase activity. This communication describes the use of this compound for the demonstration of amylase isoenzymes sep...

Five techniques for Gram staining bacteria in paraffin sections were compared on serial sections of pulmonary tissues from eight bacteriological necropsies. Brown and Hopp's method was the most satisfactory for distinguishing Gram-positive and Gram-negative bacteria. However, this method cannot be recommended as the preparations were frequently overstained, and the Gram-negative bacteria were s...

Surgery has evolved considerably in last 30-40 years. With the use of better diagnostic techniques there is a rising need for surgical procedures. There is a lag between the new technology and the delivery of clinical care with it. Currently x-rays based techniques are being used routinely during surgery. This article refreshes the history and use of fluorescence so far. It is also aimed at exp...

Aims: To extend the alkaline phosphatase-antialkaline phosphatase (APAAP) immunoenzyme single stain method to a more generally applicable double stain technique. This will allow two primary antibodies of the same isotype ofIgG and specifically the nuclear antigen bromodeoxyuridine (BRdU) to be evaluated with a cell surface antigen identifier. Method: Sequential applications of the APAAP method ...

statement of the problem: by introducing the coloring liquids, it is claimed that it is possible to make the color of frameworks fabricated from zirconium oxide extremely close to the natural tooth color. purpose: the purpose of this research was to evaluate the effect of two staining techniques on value changing in zirconia crowns. materials and method: three groups a, b, and c, each containin...

Background: The use of conventional stains including Giemsa, Wright and Leishman have become an essential tool for differential diagnosis of cells in peripheral blood. Aims: The aim of the study was to develop a new combination of Leishman-Giemsa (L&G;) stain for avian blood smears and to compare its efficacy with conventional staining methods. Methods:<...