The data presented suggest that GTP, but neither ATP nor ITP, is a substrate for the biosynthesis of the pyrrole ring of the pyrrolopyrimidine nucleoside antibiotics. GTP-&formylhydrolase from Streptomyces rimosus has been purified 240-fold; the enzyme is not constitutive and chloramphenicol markedly inhibits the biosynthesis of the pyrrolopyrimidine nucleoside antibiotic, sangivamycin, when ad...

Natural tetracycline (TC) antibiotics were the first major class of therapeutics to earn the distinction of 'broad-spectrum antibiotics' and they have been used since the 1940s against a wide range of both Gram-positive and Gram-negative pathogens, mycoplasmas, intracellular chlamydiae, rickettsiae and protozoan parasites. The second generation of semisynthetic tetracyclines, such as minocyclin...

Oxytetracycline is a polyketide antibiotic made by Streptomyces rimosus. From DNA sequencing, the gene product of otcD1 is deduced to function as a bifunctional cyclase/aromatase involved in ring closure of the polyketide backbone. Although otcD1 is contiguous with the ketoreductase gene, they are located an unusually large distance from the genes encoding the "minimal polyketide synthase" of t...

M.J. Bibb. 1983. Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J. Gen. Microbiol. 129:22572269. 6.Huang, C.H., Y.L. Yang, Y.S. Lin and C.W. Chen. 1998. The telomeres of the Streptomyces chromosomes contain conserved palindromic sequences with potentials to form complex secondary structures. Mol. Microbiol. 28:905-916. 7.Kinashi, H. and M. Shimaji-Murayama. 1991. Ph...