1. The following enzymes of the phosphorylated pathway of serine biosynthesis have been found in methanoland succinate-grown Pseudomona8 AM 1: phosphoglycerate dehydrogenase, phosphoserine-a-oxoglutarate aminotransferase and phosphoserine phosphohydrolase. Their specific activities were similar in the organism grown on either substrate. 2. A procedure for preparation of auxotrophic mutants of P...

Modulation of mitochondrial respiratory chain, dehydrogenase, and nucleotide-metabolizing enzyme activities is fundamental to cellular protection. Here, we demonstrate that the potassium channel opener diazoxide, within its cardioprotective concentration range, modulated the activity of flavin adenine dinucleotide-dependent succinate dehydrogenase with an IC50 of 32 microM and reduced the rate ...

Since the experiments of KEARNEY 1 it has been known that both particle-bound and soluble succinate dehydrogenase can be activated by its substrate succinate and by competitive inhibitors such as fumarate, malonate and phosphate. Binding at the active centre apparently brings the enzyme in an active conformation. GUTMAN et al. 2) 3 have recently reported that NADH is as effective as succinate i...

The present investigation was an attempt to evaluate the ameliorative effect of curcumin on aflatoxin-induced changes in activities of succinate dehydrogenase (SDH) and adenosine triphosphatase (ATPase) in liver and kidney of mice. Aflatoxin was obtained by growing Aspergillus parasiticus in SMKY liquid medium. Pure curcumin (97% purity) was purchased from Hi-Media Laboratories Pvt. Ltd., Mumba...

AIMS Thyroid associated orbitopathy (TAO) is an autoimmune disorder of extraocular muscles and orbital connective tissue. Identification of the principal target antigens would help the understanding of the pathogenesis of the disease and possibly lead to the development of specific therapies in the future. The purpose of this study was to measure serum antibodies against the flavoprotein subuni...

Germline mutations in the succinate dehydrogenase complex subunit D gene are now known to be associated with hereditary paraganglioma-pheochromocytoma syndromes. Since the initial succinate dehydrogenase complex subunit D gene mutation was identified about a decade ago, more than 131 unique variants have been reported. We report the case of two siblings presenting with multiple paragangliomas a...

Rat liver mitochondria isolated in 0.25 M sucrose were osmotically lysed with distilled water. The effect of osmotic lysis on mitochondrial compartmentation was monitored by following the changes in the specific Mg(++)-ATPase and the stimulation of this activity by DNP. Each resuspension in distilled water caused a progressive increase in the specific Mg(++)-ATPase and a decrease in DNP-stimula...

Production of superoxide radical during oxidation of dihydroorotate in rat liver mitochondria was not affected by antimycin A, thenoyltrifluoroacetone, or added ubiquinone but was inhibited by orotate, a product inhibitor of dihydroorotate dehydrogenase. It appears likely that superoxide is generated at the primary dehydrogenase. Dihydroorotate dehydrogenase differs from succinate dehydrogenase...