In an investigation concerned with the solubilization and characterization of membrane-bound iron-sulfur proteins of photosynthetic bacteria, succinate dehydrogenase was solubilized from Rhodospirillum rubrum chromatophores by the detergent lauryl dimethylamine oxide (LDAO) or by an alkaline washing treatment. The LDAO-solubilized enzyme was purified by a combination of DEAE-cellulose and Sepha...

Density functional theory calculations were applied to investigate 13C Chemical Shielding (CS) tensors in cryptolepine (1) and its sulfur (2) and oxygen (3) isosteres. The results showed that the CS of carbon nuclei in these compounds may be divided into three types. First, carbons type α,are those directly bonded to X (X= NH, S, O) and σ 33 shielding component of these carbons are deshield...

IscA is a key member of the iron-sulfur cluster assembly machinery found in bacteria and eukaryotes. Previously, IscA was characterized as an alternative iron-sulfur cluster assembly scaffold, as purified IscA can host transient iron-sulfur clusters. However, recent studies indicated that IscA is an iron-binding protein that can provide iron for the iron-sulfur cluster assembly in a proposed sc...

Genomic instability is related to a wide-range of human diseases. Here, we show that mitochondrial iron-sulfur cluster biosynthesis is important for the maintenance of nuclear genome stability in Saccharomyces cerevisiae. Cells lacking the mitochondrial chaperone Zim17 (Tim15/Hep1), a component of the iron-sulfur biosynthesis machinery, have limited respiration activity, mimic the metabolic res...

Warm plasma techniques are considered a promising method of tar removal in biomass-derived syngas. The fate another problematic syngas impurity—hydrogen sulfide—is studied this work. It is revealed that processing simulated with microwave results hydrogen sulfide conversion. For different gas flow rates (20–40 NLPM) and concentrations ranging from 250 ppm to 750 ppm, the conversion rate varies ...

Energy dispersive x-ray analysis was used to study the composition of certain protein body components in Cucurbita maxima cotyledons. The globoid crystal was rich in phosphorus, potassium, and magnesium. This elemental composition provides further evidence that the globoid crystal in squash cotyledon protein bodies is composed of phytin, a myoinositol hexaphosphoric acid salt of potassium and m...

Persistent microorganisms in endodontically treated teeth produce volatile sulfur compounds (VSC) such as methyl mercaptan, hydrogen sulfide, and thioether. In this retrospective study, we evaluated the ex vivo immune response of peripheral blood mononuclear cells to sulfur compounds in 354 patients with systemic diseases. These systemic findings are correlated with semiquantitative values of a...

Sulfur can be removed from benzothiophene (BT) by some bacteria without breaking carbon-carbon bonds. However, a clear mechanism for BT desulfurization and its genetic components have not been reported in literatures so far. In this study, we used comparative transcriptomics to study differential expression of genes in Gordonia terrae C-6 cultured with BT or sodium sulfate as the sole source of...

Triton-fractionated photosystem-I particles poised at -625 mV, where the two bound iron-sulfur proteins are reduced, have been studied by optical and electron paramagnetic resonance spectroscopies from 293 to 5 K. At 5-9 K, these particles exhibit two decay components with lifetimes of 1.3 and 130 msec in the laser pulse-induced absorption and electron paramagnetic resonance signal changes. Spe...

Mitochondrial cysteine desulfurase is an essential component of the machinery for iron-sulfur cluster biosynthesis. It has been known that human cysteine desulfurase that is catalytically active in vitro can be prepared by overexpressing in Escherichia coli cells two protein components of this system, the cysteine desulfurase protein NFS1 and the auxiliary protein ISD11. We report here that thi...