Although automation was introduced into biochemistry in 1957 (Skeggs, 1957), it was not until 1963 that McNeil, Helrick, and Ferrari (1963) first described a mechanized technique forABO grouping. This was followed a few months later by a method employing the AutoAnalyzer continuous flow principle by which up to 40 samples per hour could be both ABO and Rh (D) grouped (Sturgeon, Cedergren, and M...

The Technicon autoanalyzer system used for the detection of homologous human hemagglutinins has been modified for the detection of hemagglutinins to sheep red blood cells. Antibody quantitation was obtained by plotting the optical density (OD) readings of peak heights of a serially diluted reference serum versus the serum dilutions. When the OD of the peak height of an unknown sample fell withi...

The rate of the Jaffé reaction depends on the concentration of sodium hydroxide; the pseudo-first-order rate constant of the reaction, at 37 degrees C in 10 mmol/L picrate solution, is 0.004 mmol/L. We formulated an automated method to determine urinary creatinine directly without manual sample dilution. The conditions are as follows: 10 mmol/L picrate and 60 mmol/L sodium hydroxide (final conc...

This report describes an interlaboratory comparison of enzymatic serum glucose methods as currently applied in several instrumental adaptations. Four spectrophotometric, hexokinase-based methods used with the Du Pont "aca," the Abbott "Bichromatic Analyzer," the Aminco "Rotochem," and the Technicon "AutoAnalyzer ll" were compared with glucose oxidase-based methods as used with the Beckman "Gluc...

distribution of urea in the ocean and the An automated method for the determination use of 15N-labeled urea enrichment techof urea in seawater, adapted to a Technicon AutoAnalyzer, is sensitive enough to detect niques require a method that is rapid, urea at the 0.05 pg-atom urea-N liter-l level. sensitive, and convenient for use at sea. An adaptation of the diacetyl monoxime The importance of u...

The microiodometric assay of Novick for penicillinase was adapted to the Technicon Autoanalyzer system. With this automated method, it is possible to analyze almost 60 samples per hr. Two different procedures are described: in the first, the samples were incubated with substrate in the machine under standardized conditions; in the second, samples were incubated outside the Autoanalyzer and assa...

I compared enzymic methods for total serum cholesterol as used with discrete (Du Pont aca and reagent packs) and continuous-flow (Boehringer Mannheim reagents and the Technicon AuotAnalyzer I) analysis of normal, icteric, and lipemic sera. The regression equation for 24 clear, non-icteric sera was: y (continuous flow) = 0.944x (aca) + 10.69; r = 0.971, Syx +/- 53.7 mg of cholesterol per liter. ...