山麦冬属( Li r io p e Lour .)植物系百合科植物 , 中国分布有 8 种(变种) 1 ] ,分别为山麦冬( Li r io p e s p ica ta Lour .) 、湖 北 山 麦 冬 ( Li r io pe sp icata Lour . var . prol i f e ra Y . T . Ma) 、短葶山麦冬 [ Li r io p e musacar li (Decne .) Bailey] 、阔叶山麦冬 ( Li r io p e p lat y phy l la Wang et Tang) 、甘肃山麦冬 [ Li r io p e kansuensi s (Batal .) C . H . Wright] 、禾 叶山麦冬[ Li r io pe g ram ini f olia (L .) Baker]和矮 小山麦冬[ Li r io p e ...

The current standard of care for the treatment of chronic hepatitis C is pegylated interferon-α (PEG-IFNα) and ribavirin (RBV). The treatment achieves a sustained viral clearance in only approximately 50% of patients. Recent whole genome association studies revealed that single nucleotide polymorphisms (SNPs) around IL-28B have been associated with response to the standard therapy and could pre...

The Sleeping Beauty (SB) transposon system is a nonviral DNA delivery system in which a transposase directs integration of an SB transposon into TA-dinucleotide sites in the genome. To determine whether the SB transposon system can mediate stable gene expression in human T cells, primary peripheral blood lymphocytes (PBLs) were nucleofected with SB vectors carrying a DsRed reporter gene. Plasmi...

OBJECTIVE Polycythemia vera (PV) is a clonal myeloproliferative neoplasia associated with the activation of the Janus-activating kinase 2 (JAK2) mutation. The aim of this study is to identify clonal expansion of exon 12 mutations. METHODS We performed DNA sequencing of the JAK2 exon 12 after TA-cloning in JAK2-V617F-negative and JAK2-V617F-positive PV patients. RESULTS AND CONCLUSIONS We fo...

Name of Sequence. Porcine agouti-related protein (AGRP) gene. Genus and Species. Sus scrofa. Origin of Clone. Initially, a 978-bp fragment was amplified from genomic DNA using PCR primers (forward 5′-ATGCTGACCGCAGTGTTGCTGAGC-3′ and reverse 5′-CTAGGTGCGGCTGCAGGGGTTCGT-3′) designed from consensus alignment of human, bovine, and mouse DNA sequences. The PCR product was cloned into the pCR 2.1 vect...

PURPOSE To identify novel mutations in FZD4 gene that cause familial exudative vitreoretinopathy (FEVR) in Indian patients. METHODS The study was conducted on 75 subjects from 53 Indian families. These families were clinically diagnosed to have FEVR by fundus examination and fluorescein angiography. The candidate gene FZD4 was amplified from genomic DNA and PCR products were screened for muta...

DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T. Digestion of the plasmid with EcoRI confirmed the c...

PURPOSE Transmembrane CD34 glycoprotein is the most important marker for identification, isolation and enumeration of hematopoietic stem cells (HSCs). We aimed in this study to clone the cDNA coding for human CD34 from KG1a cell line and stably express in mouse fibroblast cell line NIH-3T3. Such artificial cell line could be useful as proper immunogen for production of mouse monoclonal antibodi...