The integrity of sperm progesterone (P4) receptor(s) and its response to steroid stimulation might be crucial for the maintenance of sperm fertilizing ability after cryopreservation. The aim of the current investigation was to study the effect of cryo-procedures on canine sperm P4 receptor(s). In addition, alteration of P4 receptor(s) at the molecular level and their functional integrity follow...

The objective of this study was to evaluate processing methods for frozen beef subprimals; the effects of freezing and thawing rates on tenderness, sensory properties, and retail display were evaluated. There were 6 treatments: fresh, never frozen 14 d wet aged (14D); fresh, never frozen 21 d wet aged (21D); blast frozen-fast thawed (BF); blast frozen-slow thawed (BS); conventionally frozen-fas...

A significantly higher survival rate was obtained when frozen-thawed morulae were transferred to oviducts of recipients ovulating after donors rather than to those of synchronized or earlier ovulating recipients. The highest proportion of live young, 23%, was obtained after transfer of eggs to recipients ovulating 18 h after donors. The proportion of live young (48%) was significantly increased...

The effects of Equex STM paste (Equex) and oxytocin (OT) on the in vitro quality of frozen-thawed Asian elephant sperm were investigated in the study. The viability of frozen-thawed sperm was significantly higher in the Equex-treated (1 and 2%) groups than in the control group. There were no differences in the examined sperm parameters among the control and OT-treated (0.05-5IU) groups.

In order to reduce the chance of multiple pregnancy during in-vitro fertilisation (IVF)– embryo transfer treatment, there is an increasing trend towards transferring a lower number of embryos. Surplus good-quality embryos are then transferred in frozen-thawed embryo transfer (FET) cycles. The first pregnancy after FET was reported in 1983.1 It has been estimated that in IVF programmes in which ...

BACKGROUND Artificial insemination (AI) can serve as a powerful tool to the sheep owners for making rapid genetic progress of their flock. The AI in sheep is mostly performed using fresh semen with two reasons i) lambing rate following trans-cervical AI with frozen semen is limited by the inability of frozen-thawed sperm to transit the cervix and ii) the need of circumventing the cervical barri...

In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreserva...

The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolk-based extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined a...

Results: There were no significant differences in semen quality associated with the shape of the centrifugation tube either for refrigerated (5 ̊C) or thawed semen. The g-force of centrifugation did affect semen quality. In refrigerated samples centrifuged at 250g, the percentage of sperm with normal apical ridges (NAR) was lower (P <.01) and the percentage of damaged acrosomes was higher (P <.0...

The aim of this study was to assess the effect of cooling on sperm motility before and after frozenthawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5°C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5°C for 0, 2, 7 or 24 h) after the semen wa...