In Bacillus sphaericus and other Bacillus spp., D-amino acid transaminase has been considered solely responsible for biosynthesis of D-glutamate, an essential component of cell wall peptidoglycan, in contrast to the glutamate racemase employed by many other bacteria. We report here the cloning of the dat gene encoding D-amino acid transaminase and the glr gene encoding a glutamate racemase from...

bacterial spores. The results indicate that tylosin is strongly inhibitory. Additional tests confirm the adjuvant effect of nisin and heat on spores of Bacillus stearothermophilus. These tests also show that tylosin has marked stability to heat as compared to the lesser heat stability of nisin. The ability of tylosin to prevent the outgrowth of naturally occurring thermophilic spores in a comme...

The members of the Gram-positive endospore-forming bacteria that made up the genus Bacillus have been gradually subdivided, during the last few years, into a number of new genera such as Alicyclobacillus, Aneuribacillus, Brevibacillus, Gracilibacillus, Paenibacillus, Salibacillus, Ureibacillus, and Virgibacillus. Nazina et al. in 2001 created the genus Geobacillus based around Bacillus (now Geo...

Extensive enzymatic studies have been conducted on cell-free fractions from a thermophilic bacterium designated as National Canners Association strain 2184 and tentativelv identified as Bacillus stearothermophilus Donk (1920), (Militzer et al., 1949, 1950, 1951, 1952; Georgi et al., 1951; h'Iarsh and Militzer, 1952). Preparatory to extending these investigations to other thermophilic bacteria, ...

Thermophilic bacteria are a source of bioactive compounds that have many benefits for human life. One them is as antimicrobials. This research aimed to identify and characterize the promising thermophilic bacterial isolates by analyzing their potential antimicrobial agents. with code LBKURCC were taken from collection Biochemistry Laboratory University Riau. Forty-four purified strains tested a...

Thompson, T. L. (University of Nebraska, Lincoln), and J. M. Shively. Lethal action of ribonuclease for thermophilic bacilli. J. Bacteriol 91:673-676. 1966.-Exposure of a thermophilic bacillus, NU 47, to 0.2 mug/ml of pancreatic ribonuclease resulted in a drastic reduction of viable cells. The observed lethality is thought to be associated specifically with the ribonuclease, since heat treatmen...

The production and characterization of cellulase from thermophilic strain Bacillus sp. C1AC5507 was studied. For enzyme production, sugarcane bagasse was used as carbon source. The produced carboxymethyl cellulase (CMCase) had a molecular weight around 55 kDa and its activity varied between 0.14 and 0.37 IU mL in conditions predicted by Response Surface Methodology. The optimum temperature and ...