The processing of banana into clarified juice provides an alternative in the beverages market, but further exploration related is required. Hence, this study investigated pre-treatment effect during membrane-based process on flux behaviour, fouling mechanism and quality attributes. Observation viscosity was done after crude pre-treated with 0.1 – 0.5% pectinase. Both pectinase-treated untreated...

the main objective of this study was to purify and characterize an esterase from shewanella sp f88. the enzyme was purified 41-fold and an overall yield of 21 %, using a two-step procedure, including ammonium sulfate precipitation and q-sepharore chromatography. molecular weight of the enzyme was 62.3 kda according to sds-page data. the enzyme showed an optimum activity at ph 6.5 and 58 ˚c. evo...

background: screening is a routine procedure for isolation of microorganisms which are able to produce special metabolites. purified thermostable α-amylase from bacterial sources is widely used in different industries. in this study we analyzed samples collected from three different hot springs in iran to detect any strains capable of producing thermostable α-amylase. materials and methods: hot...

A total of 520 clinical and environmental isolates of the family Micrococcaceae that fermented glucose anaerobically were tested for their ability to produce coagulase, thermostable nuclease, and deoxyribonuclease. Of these, 450 isolates coagulated rabbit plasma, produced thermostable nuclease, and were identified as Staphylococcus aureus, 447 of which produced a 3+ to 4+ clot. The remaining th...

The prokaryotic residents of the Tis solar saltern in the southeast of Iran on the shore of Oman Sea were investigated by the culture-dependent methods. Sequencing of the PCR-amplified fragments of 16S rRNA genes revealed that bacterial populations were related to Actinobacteria, Bacteroidetes, Balneolaeota, Firmicutes, and Proteobacteria. T...

A procedure for the preparation of clarified Pawpaw Juice was developed. About 750ml Pawpaw pulp was measured into 2 measuring cylinders A & B of capacity 1 litre heated to 40C, cooled to 20C. 30mls pectinase was added into cylinder A, while 30mls distilled water was added into cylinder B. Enzyme treated sample (A) was allowed to digest for 5hours after which it was heated to 90C for 15 minutes...

Porous cellulose beads were prepared through a simple, facile, and inexpensive method. The resultant microspheres exhibited good spherical shape with a diameter of 1 to 2 mm. Their morphology, pore structure, and physical properties were characterized by scanning electron microscopy, X-ray diffraction, and nitrogen adsorption. The regenerated cellulose was shown by scanning electron microscopy ...

Endophytic actinobacteria produce active metabolites, protecting plant tissues against stress. They compounds are widely used in human and veterinary pharmaceutics and agriculture, and of several enzymes with biotechnological potential. The present study characterizes the enzymatic production of endophytic actinobacteria colonizing tomato plants. Twenty-three actinobacteria isolates were tested...

Recently, it has been suggested that pectinases could be used to hydrolyze pectin in biorefineries based on pectin-rich agro-industrial wastes. However, for this to be viable, the cost of their production would need to be lowered significantly. In fact, over the last few decades, there have been many attempts to improve pectinase production by existing strains or to screen for new strains from ...

BACKGROUND Pectin is an abundant component in many fruit and vegetable wastes and could therefore be an excellent resource for biorefinery, but is currently underutilized. Fungal pectinases already play a crucial role for industrial purposes, such as for foodstuff processing. However, the regulation of pectinase gene expression is still poorly understood. For an optimal utilization of plant bio...