This paper presents a review of the literature concerning development of the stationary phases for thin-layer chromatography (TLC) in the last ten years. The silica gel remains the most important adsorbent for TLC separation. The kinetic properties of the silica-gel thin layer and the new TLC plates have been presented. Other materials used as stationary phase were alumina, zirconium oxide, Flo...

Separation of the amino acids in urine by use of thin-layer chromatography (TLC) has hitherto required that the specimen be first desalted and then chromatographed in two dimensions with at least two pairs of developing solvent systems. We wished to simplify both steps. The customary method of desalting on a column is replaced by desalting on a plate that supports a strongly acid cation-exchang...

The components of commercial sodium stearoyl lactylate (SSL), purchased in Japan, were determined and identified using thin layer chromatography (TLC) and liquid chromatography with mass spectroscopy (LC-MS). Stearoyl lactate (SL) and stearoyl-2-lactylate (SLL) were purified using TLC and silica gel chromatography to obtain standards. The results show that SSL consisted of lactic acid (8.4%), s...

The carotenoids of yellow, red and tangerine tomato fruits were separated and identified by thin-layer chromatography (TLC). β-carotene is extracted with a mixture content hexan and acetone. The various systems of adsorbents and solvents for TLC of carote and tomato carotenes were examined, and it was revealed that the most suitable TLC system for separation and identification was silica gel pl...

Objective: Honey is a natural sweet substance known for various health benefits and used in many traditional medicines dietary supplements. It contains bioactive constituents like sugars, amino acids, polyphenols, flavonoids minerals. Quality control of honey an essential part ensuring its therapeutic usage. In the present study, was analyzed by using spectroscopic approaches physicochemical me...

The illegal use of anabolics in 1989-1990 and its control in Belgium are reviewed. After a short presentation of the Belgian legislation and EEC directives, a recent hormone scandal published by a consumer magazine is related and the composition of 17 hormone cocktails found on the black market is given. The strategy of control involves screening of the main artificial anabolics in urine by rad...

The retention parameters of seven monoand nine ditetrazolium salts were investigated by reversed-phase liquid chromatography (RP-LC) using polyethylene-coated alumina support with various concentration of eluent-mixtures and by reversedphase thin-layer chromatography (RP-TLC) using the same support and eluent-mixtures. Principal component analysis (PCA) followed by non-linear mapping (NLM) and ...

Abbreviations: CYA: czapek yeast extract agar DEPT: distortionless enhancement by polarization transfer DMF: dimethylformamide EIMS: electron-ionization mass spectrometry EtOAc: ethylacetate G25N: 25% glycerol nitrate agar HMBC: heteronuclear multiple bond correlation HMQC: heteronuclear multiple quantum coherence MEA: malt extract agar MIC: minimum inhibitory concentration NMR: nuclear magneti...

Background: Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the cyst, has been investigated. Methods: The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), cyst...