Capillary zone electrophoresis provides a rapid method for the analysis of minute amounts of amino acids. A laser-based absorbance technique is used to determine phenylthiohydantoin amino acids with detection limits of 10.15 mot; separation of 20 amino acids, based on micelle modified zone electrophoresis, requires 13 minutes. The amino acid sequence of a protein is of fundamental value in the ...

For the determination of N-terminal sequence of amino acids in peptides and proteins the 1,2,4-fluorodinitrobenzene (FDNB) method (dinitrophenyl (DNP) method) of Sanger (1) has been successfully used. From a series of DNP peptides identified in partial acid hydrolysates of the DNP protein, the N-terminal sequence can be deduced (2-5). A limitation of this method is that, if an acid-labile pepti...

The purposes of this study are to determine the molt cycle of the American crayfish, Procambarus clarkit; and to quantify the amounts of the molt-inhibiting hormone (Prc-MIH) in the hemolymph and neurohemal sinus glands during the molt cycle of the American crayfish. The molt cycle was classified into six stages based on the changes in volumes of gastroliths in the stomach and ecdysteroid titer...

In preparing for metamorphosis insects store in their hemolymph and fat bodies a major nutrient reserve of 500-kDa hexamerins. At least three hexamerins serve this function in Lepidoptera, including arylphorin (ArH) and two high methionine proteins (M-MtH and V-MtH). Six day-old adults of Manduca sexta are shown here to have consumed over 99% of their pupal reserves of ArH and in the case of ma...

A structure-activity relationship study was carried out on a series of thiohydantoins and their analogues 14 which led to the discovery of 92 (MDV3100) as the clinical candidate for the treatment of hormone refractory prostate cancer.