conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...

invasive aspergillosis (ia) are a major complication in immunocompromized patients where can be serious and rapidly fatal. early diagnosis and early appropriate antifungal treatment is important in reducing mortality and morbidity. but despite many efforts to develop detection methods, the diagnosis of ia still remains challenging and current conventional methods are limited for adequate diagno...

The analysis of semen microbiota is difficult due to the lack established criteria for interpretation microbiological tests. aim study was determine stable clusters analyzed by real-time PCR in samples with normozoospermia. Semen 227 men normal spermiograms were included study. quantity total bacterial DNA and at least one group microorganisms more than 103 GE/ml 107 (41.7%) samples. Four preva...