Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis cariniipneumonia, it does not normally stain the cyst wall. For detection ofthe cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining wit...

The early diagnosis of oral cancer and precancer is important because oral cancer is usually well advanced at the time of diagnosis, having spread to regional lymph nodes in a substantial percentage of cases. The earlier the diagnosis is made, the better the prognosis. Several diagnostic methods that are used, such as stomatoscopy, toluidine blue staining, oral exfoliative cytology, histopathol...

Introduction: We report an elderly gentleman with Extramammary Paget’s disease (EMPD) treated Mohs micrographic surgery (MMS) using Toluidine blue staining intraoperatively as to detect the cells. Case Presentation: An man presented erythematous plaque on left inguinal fold which showed in-situ EMPD histopathological examination. Investigations for secondary were negative and patient was MMS. D...

Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis carinii pneumonia, it does not normally stain the cyst wall. For detection of the cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining w...

The primo vascular system (PVS), floating in lymph ducts, was too transparent to be observed by using a stereomicroscope. It was only detectable with the aid of staining dyes, for instance, Alcian blue, which was injected into the lymph nodes. Some dyes were absorbed preferentially by the PVS than the lymph wall. It remains a standing problem to know what dyes are absorbed better by the PVS tha...

The objective of this study was to analyze the effects of type-I collagen hydrogel of different concentrations on the growth and differentiation of rabbit chondrocytes. Articular cartilage from New Zealand white rabbits was harvested and cultured. Second-generation chondrocytes were collected for in vitro culture with 10, 7, and 5 mg/ml type-I collagen hydrogel, respectively (denoted as groups ...

introduction: the novel development of the (hba) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. the purpose of this study was to evaluate the correlation between hba score and histone deficiency, protamine content and dna fragmentation. methods: during april- march 2012 patients who were enrolled at shiraz-...

varicocele is one of the most common causes of primary male infertility. pilea microphylla (pm) is being used as folk medicine. this study was aimed to investigate the effects of pm in a rat model of varicocele. a total of 30 male wistar rats were divided into control, sham, varicocele, accessory varicocele and pm-treated groups. after 10 weeks of varicocele induction, sperm parameters and chro...

Cytological and cytochemical studies of green monkey kidney cells infected with SV40 virus indicated that the type of lesion produced was influenced by the multiplicity of infection and that the lesions appeared later and progressed more slowly when the inoculum was diluted. The earliest change consisted of enlargement of ribonucleoprotein-containing spherules in the nucleolus (nucleolini). Thi...