An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the Calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-o...

D URING A STUDY" 2 of variations in serum cholesterol, we observed striking changes from day to day in certain subjects. In a few individuals the change in serum cholesterol was more than 200 mg. per cent from one morning to the next. In sharp contrast , in other subjects the level of serum cholesterol seemed quite stable. Similar observations have been made by Thomas and Eisenberg.3 The tenden...

Reference intervals of total cholesterol, HDL cholesterol and non-HDL cholesterol concentrations were determined on 309 blood donors from an urban and peri-urban population of Botswana. Using non-parametric methods to establish 2.5th and 97.5th percentiles of the distribution, the intervals were: total cholesterol 2.16 - 5.52 mmol/l; non-HDL cholesterol 1.22 - 4.48 mmol/l; and HDL cholesterol 0...

recent studies have documented the importance of lipids and lipoproteins as determinants of serum total homocysteine concentrations in persons with myocardial infarction, and in healthy subjects. associations between various biological systems and total homocysteine have also been reported. however, some of these associations are poorly understood. the purpose of our study was to measure serum ...

purpose: to evaluate the association between hyperlipidemia with severity of diabetic retinopathy in patients with type i diabetes mellitus referred to the eye clinic at imam khomeini hospital, ahvaz, iran. methods: this observational cross-sectional study included 52 patients with type i diabetes mellitus. fasting blood samples for analysis of serum lipids and hba1c were obtained from all pati...