The distribution of surface sialic acid and its possible alteration after ADP-induced shape change and aggregation were studied. Gel-filtered platelets that were aggregated, disaggregated, and centrifuged showed decreased total protein and a significant decrease in sialic acid/mg protein. Platelet surface sialic acid was labeled by periodate oxidation followed by borotritiide reduction. Tritium...

BACKGROUND Milk is the sole source of nutrients for neonatal mammals and is generally considered to have co-evolved with the developmental needs of the suckling newborn. One evolutionary conserved constituent of milk and present on many glycoconjugates is sialic acid. The brain and colon are major sites of sialic acid display and together with the liver also of synthesis. METHODOLOGY/PRINCIPA...

N-Glycolylneuraminic acid (NeuGc) is distributed in most animals except humans and chickens. However, human and chicken cancerous tissues often synthesize this heterophilic sialic acid as a tumor-associated Hanganutziu-Deicher antigen [M. Naiki and H. Higashi, Adv. Exp. Med. Biol., 152: 445-456, 1982; H. Higashi et al., Cancer Res., 45: 3796-3802, 1985]. In this paper, NeuGc in human cancerous ...

Nuclei prepared from host liver and from Morris hepatomas 7777 and 7800 have been compared with respect to some of their biochemical characteristics. Several criteria were used to ensure that liver and hepatoma nuclei were of equal purity. These criteria include equal specific activity ratios (homogenate nuclei) for several marker enzymes. Phospholipids, proteins, and sialic acid content were c...

BACKGROUND The objective of this study was to compare the levels of total (TSA) and free (FSA) sialic acid in acute and chronic liver diseases. MaTERIALS AND METHODS: The serum TSA and FSA levels were determined in 278 patients suffering from acute and chronic liver diseases of different etiologies. TSA was estimated by enzymatic method and FSA by the thiobarbituric method modified by Skoza and...

Sialic acid (Neu5Ac, 1), a constituent of sialoglycoproteins1 and gangliosides,2 mediates a number of intercellular and cell-virion recognition events.3 Analogs of sialic acid are interesting as potential inhibitors for medicinally important target proteins, such as neuraminidase of the influenza virus.4 The syntheses of sialic acid and its analogs have been the subject of extensive research.5 ...

The behavior of the 2 sialidase forms present in the erythrocyte membrane was investigated in 117 subjects with type 2 diabetes mellitus versus 95 healthy controls. A significant increase of the acidic form of sialidase, which is anchored to the membrane by a glycosylphosphatidylinositol bridge, was observed in erythrocyte resealed membranes. On the contrary, the neutral form of the enzyme, the...

Concentrations of serum sialic acid, malondialdehyde (MDA), retinol, zinc, and copper were measured in 52 lactating Holstein cows. Out of them, 15 were controls, 21 mildly lame, 7 moderate lame, and 9 severely lame. The animals were housed in free-stall barns with a slurry surface and were not released for pasture feeding. Compared to control animals, the mean serum total sialic acid concentrat...

Infection by Aspergillus fumigatus, which causes the life-threatening disease invasive aspergillosis, begins with the inhalation of conidia that adhere to and germinate in the lung. Previous studies have shown that A. fumigatus conidia express high levels of the negatively charged 9-carbon sugar sialic acid, and that sialic acid appears to mediate the binding of A. fumigatus conidia to basal la...

This study was designed to study the relationship between serum nitric oxide and sialic acid in patients of diabetic nephropathy. Total 210 diabetic patients including 115 males and 95 females, suffering from diabetes and nephropathy (DN) were selected followed by informed consent and approval from institutional ethical committee. Equal number of age and sex matched normal healthy subjects were...