AIM To test the hypothesis that sudden infant death syndrome (SIDS) may be caused by toxins of commonly occurring bacteria in infants lacking developed immunity. METHODS Nasopharyngeal microbial isolates from 22 pairs of SIDS cases and healthy infants matched for age (by month), sex, and sampling time (by month) were compared for lethal toxigenicity. Crude toxin preparations were made from is...

Three naturally occurring toxigenic strains (HB-36, G-50, and HB-33), one nontoxigenic strain (HB-20), and one ultraviolet light-induced toxinless mutant (G-50 Tox) of Pseudomonas phaseolicola were examined by dye-buoyant density equilibrium centrifugation for the presence of plasmid deoxyribonucleic acid. All strains contained plasmid deoxyribonucleic acid. Comparison of the plasmid deoxyribon...

Toxin production and the presence of the toxA gene was assayed in 24 Pasteurella multocida strains isolated from pigs with pneumonia and pleuritis in the State of Rio Grande do Sul, Brazil. Toxigenicity was assessed through a mouse lethality test and a PCR amplification assay was used to obtain a 300 bp product from the toxA gene. In order to demonstrate that the amplified product corresponded ...

Although numerous workers, especially Northrop and DeKruif (1922) and Winslow, et al. (1923 and 1926), have studied the influence of electrolytes upon the electrophoretic migration velocity of bacteria by washing them free of their metabolic products and suspending them in solutions of the electrolytes, little attention has been given to the effect upon electrophoretic potential of electrolytes...

Epidemic cholera caused by toxigenic Vibrio cholerae O1 is a major health problem in several developing countries. Traditional methods for identifying V. cholerae involve cultural, biochemical and immunological assays which are cumbersome and often take several days to complete. In the present study, a direct cell multiplex PCR was developed targeting the ompW, ctxB and rfbO1 genes for confirma...

General characteristics of the group; methods of study..................... 4 Bovine pleuropneumonia, and the etiological agent........................ 13 Agalactia of sheep and goats, and the etiological agent................... 16 Pleuropneumonia-like microorganisms of dogs; their relation to canine distemper........................................................... 18 Pleuropneumonia-like m...