Quinine is a promising natural product building block for polymer-based nucleic acid delivery vehicles as its structure enables DNA binding through both intercalation and electrostatic interactions. However, studies exploring the potential of quinine-based polymers applications (transfection) are limited. In this work, we used hydroquinine-functionalized monomer, HQ, with 2-hydroxyethyl acrylat...

We aimed to evaluate the regulatory effects of miR-148a-3p on stem cell osteogenic differentiation and enamel development by targeting runt-related transcription factor 2 (RUNX2) E-cadherin, respectively. TargetScan software was utilized predict binding sites between marker gene RUNX2 or E-cadherin. The changes in expression during epidermal cells were detected. After transfection with mimics i...

BACKGROUND Currently, cationic liposome has become the commonly used vehicles for gene transfection. Furthermore, one of the most significant steps in microRNAs expression studies is transferring microRNAs into cell cultures successfully. In this study we aim to approach the feasibility of transfection of cervical cancer cell lines mediated by liposome and to obtain the optimized transfection c...

The aim of the present study was to assess the effects of sprouty homolog 2 (SPRY2) gene regulation by miR-21 on the occurrence, development and tumor metastasis in multiple myeloma (MM). The miR-21 expression lentiviral vector (LV)-anti-miR-21 and a liposome transfection method were used to screen MM cell lines with stable silent SPRY2. Real-time quantitative polymerase chain reaction (PCR) an...

65 2.1 Material and Methods 66 2.1.1 Plasmid and cloning 66 2.1.2 Cell culture and transfection 66 2.1.3 Assessment of cell viability 67 2.1.4 Assessment of Neurite process length and branching point. 68 2.1.5 Ras Pull-Down Assay 68 2.1.6 Generation of Human LRRK2 Transgenic Flies. 69 2.1.7 Western Blot Analysis. 70 2.1.8 Immunoprecipitation. 71 2.1.9 Optical Neutralization Technique. 71 2.1.10...

Ultrasound and poly(ethylenimine) (PEI) have each separately been shown to increase DNA transfection efficiency. This study tested the hypothesis that the combination of ultrasound and PEI can have a synergistic effect to increase DNA transfection. This in vitro study assessed transfection efficiency of two different DNA plasmids encoding green fluorescent protein and firefly luciferase in two ...

A wide variety of bioinformatic tools have been described to characterize potential transcriptional regulatory mechanisms based on genomic sequence analysis and microarray hybridization studies. However, these regulatory mechanisms are still experimentally verified using transient transfection methods. Current transfection methods are limited both by their large scale and by the low level of ef...

Objective : The anti-tumor effect of the beta-adrenergic receptor antagonist propranolol in breast cancer is well known; however, its activity glioblastoma not well-evaluated. Notch-Hes pathway known to regulate cell differentiation, proliferation, and apoptosis. We investigated human lines, role Notch Hes signaling this process.Methods performed immunohistochemical staining on 31 surgically re...

background: baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. the presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. methods: the aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

The efficiency of the transfection of a plasmid DNA encoding a galactosidase promoted by a series of pyridinium lipids in mixtures with other cationic lipids and neutral lipids was assessed in CHO-K1 cells. We identify key molecular parameters of the lipids in the mixture - clog P, lipid length, partial molar volume - to predict the morphology of the lipid-DNA lipoplex and then correlate these ...