The growth inhibitory effects of gallium on a murine and human B-cell line were studied using two different serum-free culture systems: (a) ferric citrate medium containing 500 microM iron and (b) transferrin medium containing 5 micrograms/ml of iron-saturated transferrin (0.125 microM iron). For the human cell line in ferric citrate medium, 50% growth inhibition achieved in the presence of tra...

The present study was conducted to examine different dietary levels of iron (FeSO4) on some haematological parameters of ship sturgeon, Acipenser nudiventris including red blood cells (RBC), white blood cells (WBCs), differential WBCs, hematocrit (Hct), Hemoglobin (Hb), the mean corpuscular hemoglobin (MCH) and the mean corpuscular hemoglobin concentration (MCHC), the mean corpuscular volume (M...

Hereditary hemochromatosis (HH) is a genetic iron overload disease, in the majority of cases associated with homozygosity for the C282Y mutation of the HFE gene. In spite of this genetic homogeneity, there is a great clinical heterogeneity among HH patients. Low CD8(+) lymphocyte numbers have been associated with a more severe expression of iron overload in HH patients, and in experimental mode...

A preparation of microvillous membrane vesicles from human placental syncytiotrophoblast binds transferrin to specific transferrin receptors. Transferrin binding to placental receptors is rapid, saturable, reversible, and specific. Approximately 2.5 X 10(13) receptors are present per milligram of membrane protein; the apparent association constant of transferrin for the placental receptor is 2....

The transferrin receptor of the human reticulocyte was isolated by two different immunoaffinity procedures. These included indirect immunoprecipitation with a transferrin/anti-transferrin complex and direct immunoprecipitation with antiserum to purified transferrin receptor from placentae. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the receptor isolated from reticulocytes reve...

Transferrin has been considered to be an essential requirement for hematopoietic cell proliferation in culture. We have isolated two cloned lymphoma cell lines, SL 12.1 and SL 12.4, which grow and adapt in serum-free medium without added transferrin. Antibody to the transferrin receptor blocks the growth of these cells. We have also demonstrated that transferrin-free condi tioned medium from th...

We have used a monoclonal antibody against the murine transferrin receptor to study the expression of the transferrin receptor on the hematopoietic progenitor cells (BFU-E, CFU-E, and CFU-C) present in mouse bone marrow. Elutriation and cell-sorting data are consistent with the hypothesis that most CFU-E are transferrin receptor positive while most BFU-E express much less transferrin receptor. ...

Rabbit transferrin in vitro is shown to load ferrous iron at random on its specific binding sites. The release of iron to reticulocytes is shown to be an all-or-none phenomenon. The two monoferric transferrins have similar in vivo plasma iron clearance rates and tissue distribution. Diferric transferrin, while giving a similar tissue distribution of radioiron, has a plasma iron clearance rate a...

Three types of asialo-transferrin were obtained from immunologically pure human transferrin by chromatography on DEAE-cellulose, followed by desialylation and affinity chromatography on a column of the immobilized asialo-glycoprotein-binding hepatic lectin from rabbit liver. Of the asialo-transferrins, type 1 was derived from the principal DEAE-cellulose chromatographic component of transferrin...

The iron regulatory hormone hepcidin is transcriptionally up-regulated in response to iron loading, but the mechanisms by which iron levels are sensed are not well understood. Large-scale genetic screens in the zebrafish have resulted in the identification of hypochromic anemia mutants with a range of mutations affecting conserved pathways in iron metabolism and heme synthesis. We hypothesized ...