OBJECTIVE To estimate the accuracy of the clinical diagnosis of the three most common causes of acute vulvovaginal symptoms (bacterial vaginosis, candidiasis vaginitis, and trichomoniasis vaginalis) using a traditional, standardized clinical diagnostic protocol compared with a DNA probe laboratory standard. METHODS This prospective clinical comparative study had a sample of 535 active-duty Un...

Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted disease (STD) in the world. The diagnosis is based on wet mount preparation and direct microscopy on fixed and stained clinical specimens. The aim of this study was to compare the performance of different fixing and staining techniques used in the detection of T. vaginalis in urine. Th...

The protozoan flagellate Trichomonas vaginalis is responsible for human trichomoniasis, one of the most widespread sexually transmitted diseases in the world. Several methods are currently used for laboratory diagnosis, including direct microscopic observation, cell culture, immunological techniques, and more recently, DNA probing and gene amplification. This report describes an in situ hybridi...

BACKGROUND Trichomonas vaginalis, which affects at least 170 million individuals globally, may increase the risk of transmission of HIV and predispose pregnant women to premature rupture of membranes and early labour. OBJECTIVE To more clearly define the epidemiology of trichomoniasis and to develop a mathematical model of disease transmission dynamics in order to explore various treatment st...

Trichomonas vaginalis was originally considered a commensal organism until the 1950s when the understanding of its role as a sexually transmitted infection (STI) began to evolve. Trichomoniasis has been associated with vaginitis, cervicitis, urethritis, pelvic inflammatory disease (PID), and adverse birth outcomes. Infection with T vaginalis could have an important role in transmission and acqu...

A study on Trichomoniasis was conducted to determine the prevalence, symptoms and factors that promote the transmission of Trichomonas vaginalis among women of Ikwuano in Abia State using high vaginal swab and urine samples. A total of 600 women aged between 14-60years were examined and 112 (18.67%) were infected with Trichomoniasis. The highest prevalence of Trichomonas vaginalis infection (20...

Trichomonas vaginalis, a human urogenital tract parasite, is capable of surviving in the male microenvironment, despite of the presence of Zn . Concentrations > 1.6 mM of Zn have a trichomonacidal effect; however, in the presence of <1.6 mM Zn , several trichomonad proteins are upor down-regulated. Herein, we analyzed the proteome of a T. vaginalis male isolate (HGMN01) grown in the presence of...

Using a whole-cell antigen antibody to Trichomonas vaginalis was measured by an enzyme-linked immunosorbent assay (ELISA). IgG antibody was found in sera from only three of 99 children under 12 years of age. In contrast, serum IgG or IgM antibody or both were detected in 80.4% of women who had vaginal trichomoniasis and in 13.7% of uninfected women. Although antibody was found in cervical and v...

The study assessed the epidemiology of Trichomonas vaginalis among 200 pregnant women aged between 15-45 years attending antenatal care in three private hospitals Idiroko, Ipokia Local Government Area Ogun State, Nigeria. Direct wet mount microscopy and culture were used on High Vaginal swabs collected from consented participants after administration questionnaire to collect their knowledge, at...

Trichomonas vaginalis is a pathogen that triggers severe immune responses in hosts. T. vaginalis α-actinin 2, Tvα-actinin 2, has been used to diagnose trichomoniasis. This study was undertaken to examine the role of Tvα-actinin 2 as an antigenic molecule to induce immune responses from humans. Western blot analysis using anti-Tvα-actinin 2 antibodies indicated its presence in the secreted prote...