Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized confrontation assay. By this we were able to visualize metabolite distribution...

The antagonistic potentialities of seven species native Trichoderma viz., T. atroviride, koningiopsis, ovalisporum, Hypocrea lixii, harzianum, asperellum and harzianum were evaluated in vitro against Cochliobolus heterostrophus causing maydis leaf blight maize. current study dual culture assay, revealed the percentages mycelial growth inhibition C. by atroviridae, asperellu 80.4%, 83.80%, 82.85...

BACKGROUND There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw material uses for the production of ethanol second generation (2G). New cellulol...

The antagonistic potential of seven species native Trichoderma against Rhizoctonia solani f.sp. sasakii were evaluated in dual culture method which includes T. asperellum (KU933476), koningiopsis (KU904460), Hypocrea lixii (KX0113223), harzianum (KU933471), ovalisporum (KU904456), (KU904458), atroviridae (KU933472). Among them showed highest inhibition percentage (78.09%), followed by (70.95%),...

Sequences of the internal transcribed spacer region 1 (ITS1) of the ribosomal DNA were used to determine the phylogenetic relationships of species of Trichoderma sect. Pachybasium. To this end, 85 strains-including all the available ex-type strains-were analyzed. Parsimony analysis demonstrated that the section is nonmonophyletic, distributing the 85 strains among three main groups that were su...

In this study Trichoderma atroviride was selected as over producer of chitinase enzyme among 30 different isolates of Trichoderma sp. on the basis of chitinase specific activity. From this isolate the genomic and cDNA clones encoding chit33 have been isolated and sequenced. Comparison of genomic and cDNA sequences for defining gene structure indicates that this gene contains three short introns...