Cells of human buccal epithelium of 6 male donors were exposed to microwave radiation (frequency f=36,64 GHz, power density E=10, 100, and 400 µW/cm 2). Exposure time in all experiments was 10 seconds. Heterochromatin was stained by 2% orcein in 45 % acetic acid. The stainability of cells with trypan blue (0,5 %) and indigocarmine (5 mM) after 5 min of staining was investigated. Irradiation ind...

Different applications of trypan blue (TB) for intraocular surgery have been reported, with very high levels of safety and efficacy. We describe the use of TB as an alternative vital dye for staining the ocular surface to assess the integrity of superficial cell layers of the cornea and the surface environment. This facilitates the diagnosis of various ocular surface disorders, including screen...

of cancer with the hope of developing useful diag nostic and therapeutics agents. The aim in such investigations has been to find a dye which, in the intact animal, stains living tumor cells without staining surrounding tissue. A large number of acid dyes, especially in the azo series, has been rather intensively investigated for such a purpose. From these investigations there has resulted gen ...

Although the potency of trypan blue as a teratogenic agent in rodents has been known for almost twenty years the mechanism of its action remains obscure. The dye produces a variety of biological effects (see review by Beck & Lloyd, 1966) and most of these have at some time been suggested as possibly relevant to its teratogenicity. The investigation of such correlations would clearly be assisted...

Introduction: Thyroid cancer is the most common endocrine malignancy tumor, and its incidence is increasing. Chemotherapy drugs such as Paclitaxel (PTX) are common treatment for cancer, but they have many side effects. Plants are as a source of anticancer agents.  The purpose of this study was the assessment of cytotoxic effects of hydro-alcoholic extract in comparison with those of Paclitaxel ...

Resorbing oocytes are heavily stained by trypan blue injected into the haemolymph; this serves as a basis for a quick and convenient method for measuring the degree of resorption. Oocytes in the beginning of their development are most susceptible towards resorptive tendencies. The uptake of trypan blue by normally developing oocytes is proportional to the oocyte surface. From 'double-marker' ex...

Schistosoma mansoni eggs are classified, according to morphological characteristics, as follows: viable mature and immature eggs; dead mature and immature eggs, shells and granulomas. The scope of this study was to compare the staining characteristics of different morphological types of eggs in the presence of fluorescent labels and vital dyes, aiming at differentiating live and dead eggs. The ...

introduction: the novel development of the (hba) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. the purpose of this study was to evaluate the correlation between hba score and histone deficiency, protamine content and dna fragmentation. methods: during april- march 2012 patients who were enrolled at shiraz-...