Abstract Background Congenital heart disease is a leading cause of death in newborns and infants. The feasibility fetal cardiac surgery linked to extracorporeal circulation (ECC); therefore, cardioplegic solutions need be effective long-lasting. Methods Eighteen pregnant sheep were divided into an ECC-only group, St. Thomas’ Hospital solution (STH1) group (STH group), HTK preservation (Custodio...

Organotypic cultures of neuronal tissue were first introduced by Hogue in 1947 (1,2) and have constituted a major breakthrough in the field of neuroscience. Since then, the technique was developed further and currently there are many different ways to prepare organotypic cultures. The method presented here was adapted from the one described by Stoppini et al. for the preparation of the slices a...

The aim of the study was to evaluate protective effects of exogenous leptin on ischemia/reperfusion (I/R)-induced injuries to the urinary bladder tissue and to investigate the effect on tumor necrosis factor alpha (TNF-alpha) levels and apoptotic cells during I/R injury. Bladder I/R injury was induced by abdominal aorta occlusion by ischemia for 45 min, followed by 60 min of reperfusion in rats...

The clinical and experimental postcardiac arrest treatment has not reached therapeutic success. The present study investigated the effect of PD98059 (PD) in rats subjected to cardiac arrest (CA)/cardiopulmonary resuscitation (CPR). Experimental rats were divided randomly into 3 groups: sham, CA, and PD. The rats except for sham group were subjected to CA for 5 min followed by CPR operation. Onc...

BACKGROUND The aim of the present study was to define the effect of apoptosis on sperm quality and function. METHODS The apoptotic features in sperm were assessed in 60 subfertile subjects, using Annexin-V staining for phosphatidylserine (PS) externalization and Tdt-mediated dUTP nick end labelling (TUNEL) assay for DNA fragmentation. RESULTS On average, about 45% of the sperm were found to...

We developed and characterized a mouse model of elevated intraocular pressure (IOP) to investigate the underlying cellular and genetic mechanisms of retinal ganglion cell (RGC) death. IOP was unilaterally increased in C57BL/6J mice by photocoagulation of the episcleral and limbal veins. IOP was measured using an indentation tonometer. RGC survival was measured by retrograde labeling using DiI a...

Abstract The incidence rate of insomnia is increasing, but the mechanism it remains unclear. Warming Yang Strategy (WY) a kind Traditional Chinese Medicine, and proved to be effective in treating patients. animal was established with chlorophenylalanine (PCPA). Morris water maze open field test were performed evaluate influence WY on neurological recovery rats. TUNEL staining flow cytometry use...

OBJECTIVE To validate the ability of (99m)Tc-Annexin V to visualize early stage of glucocorticoid-induced femoral head necrosis by comparing with (99m)Tc-MDP bone scanning. METHODS Femoral head necrosis was induced in adult New Zealand white rabbits by intramuscular injection of methylprednisolone. (99m)Tc-Annexin scintigraphy and (99m)Tc-MDP scans were performed before and 5, 6, and 8 weeks ...

OBJECTIVE To investigate the histopathological and immunohistochemical properties of degenerative changes in the ligamentum flavum of the cervical spine with calcium crystal deposition. METHODS Sections of the calcified ligamentum flavum harvested from 26 patients who required cervical decompression were examined by scanning electron microscopy (SEM), energy dispersive X-ray microanalysis, im...

DIHS/DRESS is a severe adverse drug reaction characterized by persistent eruption associated with reactivation of herpesviruses. In acute phase the disease, purpura occasionally observed mainly in limbs. We recently demonstrated that area lower limbs correlates severity as assessed score. However, pathogenesis has not been examined. collected four patients DIHS and two erythema multiforme (EM)....