Chair of Physiology, School of Pharmacy and Biochemistry, University of Buenos Aires (UBA), Argentina. IQUIMEFA-CONICET Scholar at the University of Buenos Aires. Undergraduate Teaching Assistant, Chair of Physiology, School of Pharmacy and Biochemistry, UBA Undergraduate Teaching Assistant, Chair of Physiology, School of Pharmacy and Biochemistry, UBA Undergraduate Teaching Assistant, Chair of...

Ddi1/Vsm1 is an ubiquitin receptor involved in regulation of the cell cycle and late secretory pathway in Saccharomyces cerevisiae. Ddi1 possesses three domains: an NH(2)-terminal ubiquitin-like domain (UBL), a COOH-terminal ubiquitin-associated domain (UBA), and a retroviral aspartyl-protease domain (RVP). Here, we demonstrate the domains involved in homodimerization, checkpoint regulation, lo...

Although Ddi1-like proteins are conserved among eukaryotes, their biological functions remain poorly characterized. Yeast Ddi1 has been implicated in cell cycle regulation, DNA-damage response, and exocytosis. By virtue of its ubiquitin-like (UBL) and ubiquitin-associated (UBA) domains, it has been proposed to serve as a proteasomal shuttle factor. All Ddi1-like family members also contain a hi...

Abbreviations used are: AAA ATPase: ATPase associated with various cellular activities; VCP: valosin-containing protein; ER: endoplasmic reticulum; ERAD: ERassociated degradation; NZF: Npl4 zinc finger; UBA domain: Ubiquitin-associated domain; GST: glutathione S-transferase; HA: hemagglutinin; IB: immunoblotting; IP: immunoprecipitation; RNAi: RNA interference; siRNA: small interference RNA; RI...

A new route to the synthesis of triazole-linked ubiquitin dimers (diUbs) as structural analogs of the seven diUbs is reported. Binding studies with the Lys48-specific UBA domain of the Mud1 protein suggest that they represent functionally suitable surrogates of their native counterparts linked by an isopeptide bond.

Semana Semana de la de la Matem Matem á á tica tica Departamento Departamento de de Matem Matemá ática tica FCEyN FCEyN – – UBA UBA

UBDs [Ub (ubiquitin)-binding domains], which are typically small protein motifs of <50 residues, are used by receptor proteins to transduce post-translational Ub modifications in a wide range of biological processes, including NF-κB (nuclear factor κB) signalling and proteasomal degradation pathways. More than 20 families of UBDs have now been characterized in structural detail and, although ma...