A sensitive and selective method is needed to analyse in situ produced vitamin B12 in plant-based materials, potential new dietary sources of vitamin B12. A UHPLC/UV method was developed and validated for the determination of human active vitamin B12 in cell extracts of Propionibacterium freudenreichii subsp. shermanii and after immunoaffinity purification in extracts of cereal matrices ferment...

The authors are grateful to Dr. Aygul Balcioglu, Research Scientist at MIT for providing fresh microdialysis samples for these experiments. Overview Purpose: To develop an Ultra High Performance Liquid Chromatography (UHPLC) method to resolve the major biogenic amine and their acid metabolites in a microdialysis sample collected from the rat brain with improved throughput using the Thermo Scien...

Abbreviations: UHPLC: Ultra-High-Performance Liquid Chromatography; HESI: Heated Electrospray Ionization; MS: Mass Spectrometry; MS/ MS: Tandem Mass Spectrometry; BCS: Biopharmaceutics Classification System; FDA: Food and Drug Administration; PK: Pharmacokinetic; IS: Internal Standard; m/z: mass-to-charge ratio; Cmax: Peak Plasma Concentration of Drug; Tmax: Time required to reach the peak plas...

Coupling UHPLC with accurate-mass Q-TOF mass spectrometry, combined with multivariate statistical analysis, has been demonstrated to be a promising tool for profiling whiskey. Using this technique, sets of compounds were identified that enabled discrimination among whiskey types, bourbon producers, and whiskey age.

BACKGROUND Vitamin B1 (thiamine-diphosphate) and B6 (pyridoxal-5'phosphate) are micronutrients. Analysis of these micronutrients is important to diagnose potential deficiency which often occurs in elderly people due to malnutrition, in severe alcoholism and in gastrointestinal compromise due to bypass surgery or disease. Existing High Performance Liquid Chromatography (HPLC) based methods inclu...

BACKGROUND Fuzi-Gancao herb couple is one of the commonly used herb couples involved in the traditional Chinese medicine formulations, with radix aconiti lateralis (Fuzi in Chinese) and radix glycyrrhizae (Gancao in Chinese) as a ratio of 1:1. Alkaloids and flavonoids were considered as the main active ingredients of Fuzi-Gancao herb couple. However, no analytical methods have been reported to ...

Goal To develop an efficient analytical method to perform robust and reproducible translational proteomics research. The method described here includes UHPLC separations, data acquisition using DIA strategies, and efficient, automated data processing designed to rapidly and confidently credential proteins for translation into subsequent, more targeted experimental methods for verification and v...

This work was initiated to develop a simple, quick, and reliable UHPLC-PDA-MS method identify chemical markers for authentication compare fruit samples of four elder species, Sambucus nigra, S. ebulus, canadensis, cerulea. The UHPLC showed an adequate separation 11 compounds including anthocyanins, phenolic acids, flavonoids in berries the species. Quantification, detection limits, precision, a...