In this series of two papers, 192 doping agents belonging to the classes stimulants, narcotics, cannabinoids, diuretics, β2-agonists, β-blockers, anabolic agents, and hormone metabolic modulators were investigated, with aim assess benefits limitations ion mobility spectrometry (IMS) in combination ultra-high performance liquid chromatography (UHPLC) high resolution mass (HRMS) anti-doping analy...

The aim of this work was to expand the applicability range UHPSFC series synthetic and commercialized peptides. Initially, a screening different column chemistries available for analysis performed, in combination with additives either basic or acidic nature. an additive (13 mM TFA) stationary phase (Torus DEA 2-PIC) found be best six peptides possessing acidic, neutral isoelectric points. Secon...

Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range of food and feedstuff. To date, the data on its conjugates via glucuronidation request clarification and consolidation. In the present study, the combined approaches of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution mass spectrometry (HRMS) and ...

The Agilent 1260 Infi nity Hybrid SFC/UHPLC System offers a fast, reliable, green, robust, and cost-effective solution by coupling the advantages of HPLC and the benefi ts of supercritical fl uid chromatography (SFC). This Application Note describes the conversion of the United States Pharmacopeia (USP) normal-phase HPLC method for cholecalciferol assay to an SFC method using serially coupled c...

An UHPLC-PDA-ESI/HRMS(n) profiling method was used to identify the glucosinolates and flavonoids of Rorippa indica (Cruciferae), a wild vegetable and Chinese herb used to treat cough, diarrhea, and rheumatoid arthritis. Thirty-three glucosinolates, more than 40 flavonol glycosides, and 18 other phenolic and common organic compounds were identified. The glucosinolates and polyphenols were separa...

A sensitive and selective method is needed to analyse in situ produced vitamin B12 in plant-based materials, potential new dietary sources of vitamin B12. A UHPLC/UV method was developed and validated for the determination of human active vitamin B12 in cell extracts of Propionibacterium freudenreichii subsp. shermanii and after immunoaffinity purification in extracts of cereal matrices ferment...

The authors are grateful to Dr. Aygul Balcioglu, Research Scientist at MIT for providing fresh microdialysis samples for these experiments. Overview Purpose: To develop an Ultra High Performance Liquid Chromatography (UHPLC) method to resolve the major biogenic amine and their acid metabolites in a microdialysis sample collected from the rat brain with improved throughput using the Thermo Scien...

Abbreviations: UHPLC: Ultra-High-Performance Liquid Chromatography; HESI: Heated Electrospray Ionization; MS: Mass Spectrometry; MS/ MS: Tandem Mass Spectrometry; BCS: Biopharmaceutics Classification System; FDA: Food and Drug Administration; PK: Pharmacokinetic; IS: Internal Standard; m/z: mass-to-charge ratio; Cmax: Peak Plasma Concentration of Drug; Tmax: Time required to reach the peak plas...

Coupling UHPLC with accurate-mass Q-TOF mass spectrometry, combined with multivariate statistical analysis, has been demonstrated to be a promising tool for profiling whiskey. Using this technique, sets of compounds were identified that enabled discrimination among whiskey types, bourbon producers, and whiskey age.