Three turbidometric methods and one method using ultraviolet spectrophotometry for estimating total cerebrospinal fluid protein have been examined. The necessity for preliminary dialysis renders the ultraviolet method unsuitable for routine use. The turbidometric method of Meulemans (1960) using a sulphosalicylic acid-sodium sulphate precipitating fluid is better than a method using sulphosalic...

Montelukast sodium is well known pharmaceutically for its action as leukotriene antagonist and reliving symptoms associated with asthma available in the market tablet, chewable powder. The aim of this study was to develop newly simple selective ultraviolet spectrophotometry (UV) method daily routine analysis quality control department. UV developed wavelength at 287.0 nm. This effectively appli...

Smith-Lemli-Opitz syndrome (SLOS) is an autosomal recessive disorder due to an inborn error of cholesterol metabolism, characterized by congenital malformations, dysmorphism of multiple organs, mental retardation and delayed neuropsychomotor development resulting from cholesterol biosynthesis deficiency. A defect in 3 -hydroxysteroid-delta7-reductase (delta7-sterol-reductase), responsible for t...

We present the results of a detailed analysis of the noise behavior of two CCD spectrometers in common use, an AvaSpec-3648 CCD UV spectrometer and an Ocean Optics S2000 Vis spectrometer. Light sources used include a deuterium UV/Vis lamp and UV and visible LEDs. Common noise phenomena include source fluctuation noise, photoresponse nonuniformity, dark current noise, fixed pattern noise, and re...

We present absolutely calibrated far-ultraviolet spectrophotometry of the quasar 3C 273 covering the 900–1800 Å range. Our ∼ 3 Å resolution spectra were obtained with the Hopkins Ultraviolet Telescope during the Astro1 mission in December 1990 and during the Astro-2 mission in March 1995. Both spectra exhibit a change in slope near the Lyman limit in the quasar rest frame. At longer UV waveleng...

This work deals with the simultaneous estimation of metformin hydrochloride and pioglitazone hydrochloride in a bilayered tablet dosage form, without prior separation by two techniques. The methods employed are derivative spectrophotometery and Q analysis. The absorption maxima at 231 nm and 269 nm were used for the estimation of metformin and pioglitazone, respectively. Both the drugs and thei...

A rapid method based on UV spectrophotometry is described for simultaneous determination of salicylates and barbiturates in serum. While barbiturates have no absorbance above 280 m and extracted normal serum has insignificant absorbance, salicylate has a maximal absorbance peak at 296 mjh, which is proportional to concentration. The barbiturate concentration can be calculated from absorbance di...

Typically, the first thing one wants to know about a DNA preparation is its concentration and purity. Both can be determined by measuring the absorption of ultraviolet light. DNA absorbs UV more or less strongly depending upon the wavelength. We will be using a NanoDrop spectrophotometer, which takes measurements at wavelengths of 260 and 280 nm, and in addition determines an absorption spectru...

Malignant tissues are distinguished from other types of tissues by their capacity for relatively unlimited and uncontrolled growth in the organism. The reason why neoplastic tissues grow continually, in contrast to embryonic tissues which grow only up to a certain point before they begin to differentiate, is a fundamental problem of cancer research. A study of the synthetic mechanisms of the ce...

A proposed heat precipitation and thrombin interaction technic for fibrinogen isolation has been compared with a sulfite fractionation technic. All three methods exhibit a high degree of correlation. Fibrinogen, which has been separated from plasma by these technics, may be quantitated by the colorimetric modified biuret and phenol-biuret reaction. For direct measurement of fibrinogen, heat pre...