BACKGROUND Mesenchymal stem cells (MSCs) have a capacity for self-renewal and multi-potential differentiations. These cells are considered powerful sources for cell therapy in regenerative medicine and tissue engineering. The cells can be isolated from various tissues; however, harvesting from human umbilical cord and amniotic membrane is easy and accessible source. OBJECTIVE To isolate and c...

BACKGROUND A method for qualitative detection of 57 drugs and metabolites in umbilical cord tissue using liquid chromatography time-of-flight (TOF) mass spectrometry is described. METHODS Results from 32 deidentified positive specimens analyzed by an outside laboratory using "screen with reflex to confirmation" testing were compared with TOF results. In addition, 57 umbilical cord tissue spec...

objective: to compare the maternal and umbilical cord serum selenium concentrations in low and normal birth weight neonates. materials and methods: a case-control study was carried out in vali-asr and akbarabadi hospitals (jan. to dec. 2013). two groups; case group; 91 mothers who delivered a low birth weight (lbw) neonate and control group; 86 subjects who delivered a normal birth weight neona...

Human umbilical cord-derived mesenchymal stem/stromal cells (UMSCs) demonstrate great therapeutic potential in regenerative medicine. The use of UMSCs for clinical applications requires high quantity and good quality usually by vitro expansion. However, the heterogeneity characteristics cultured cognate human cord tissue at single-cell resolution remain poorly defined. In this study, we created...

Widespread use of human umbilical cord cells for cardiovascular tissue engineering requires production of large numbers of well-characterized cells under controlled conditions. In current research projects, the expansion of cells to be used to create a tissue construct is usually performed in static cell culture systems which are, however, often not satisfactory due to limitations in nutrient a...

Background: In this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. Materials and Methods: Human umbilical cord blood-derived mesenchymal stem cells were first cultured in Dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incu...

Background: The timing of umbilical cord clamping may affect the need to bloodtransfusion and other morbidities of preterm infants. This study aimed to compare three different cord clamping timing (immediate cord clamping, delayed cord clamping and umbilical cord milking) in preterm infants delivered by cesarean section (CS). <em...

Background & Aims: Maternal anemia in pregnancy is a considerable risk factor for both mother and fetus. Growth retardation, intra uterine fetal death, maternal cardiovascular symptoms and reduction in blood supply are among outcomes of maternal anemia. Since there are different reports about the relationship between low maternal hemoglobin level and low birght weight, the precent study was des...

OBJECTIVE A major shortcoming in contemporary congenital heart surgery is the lack of viable replacement materials with the capacity of growth and regeneration. Here we focused on living autologous patches engineered from human umbilical cord derived fibroblasts and endothelial progenitor cells (EPCs) as a ready-to-use cell source for paediatric cardiovascular tissue engineering. METHODS EPCs...

Immunoreactive plasminogen activators were studied in tissue sections using a peroxidase method and monospecific antibodies to tissue plasminogen activator produced by a melanoma. Tissue plasminogen activator reactivity was found in skin melanomas and in endothelial and smooth muscle cells of arteries and veins. Vessels of the umbilical cord showed higher reactivity than peripheral vessels. Onl...