Orotidine 5'-monophosphate decarboxylase from Plasmodium falciparum (PfOMPDC) catalyses the final step in the de novo synthesis of uridine 5'-monophosphate (UMP) from orotidine 5'-monophosphate (OMP). A defective PfOMPDC enzyme is lethal to the parasite. Novel in silico screening methods were performed to select 14 inhibitors against PfOMPDC, with a high hit rate of 9%. X-ray structure analysis...

Most node localization algorithms for wireless sensor network (WSN) are only applicable to twodimensional networks. However, in most cases, nodes are placed in three-dimensional (3D) terrains, such as forests, oceans, etc. In this paper, a range-based 3D localization method is put forward based on time-of-arrival (TOA) estimation of ultra wideband signal using unitary matrix pencil (UMP) algori...

Complex U is composed of orotate phosphoribosyltransferase (EC 2.4.2.10) and orotidine-5’-phosphate decarboxylase (EC 4.1.1.23), the last two enzymes of the de nouo pathway for pyrimidine biosynthesis. Since the two enzymes have proved inseparable so far, the equilibrium constant for the phosphoribosyltransferase activity has been determined under conditions where the decarboxylase activity was...

The homopolymer complex, (dA, .dT,), will direct the synthesis of r(A),, or r(U),,, or both, in the presence of highly purified RNA polymerase from iVIicrococcus Zysodeikticus. When either ‘GATP or 3H-UTP is present with saturating amounts of (dA, . dT,), AMP incorporation is considerably greater than UMP incorporation. This difference appears to reflect a product inhibition which is observed o...

Sistem pendukung keputusan seleksi beasiswa yang berjalan saat ini di Universitas Muhammadiyah Purworejo (UMP)menerapkan metode Simple Additive Weighting (SAW). Berdasarkan pengujian sistem, tingkat akurasi sistem sebesar 80%. Hasil dirasa kurang efisien, sehingga perlu ditingkatkan, agar hasil pemilihan Lazizmu lebih optimal. Tujuan penelitian yaitu membandingkan beberapa metode, antara lain (...

Orotidine-5'-monophosphate decarboxylase (OD-Case) catalyzes the conversion of orotidine 5'-monophosphate to UMP. In mammals, ODCase is present as part of a bifunctional protein which also contains orotate phosphoribosyltransferase; the preceding enzyme in the de novo UMP biosynthetic pathway. We have isolated a plasmid (pMEJ) which contains a cDNA for the ODCase domain of UMP synthase. Inserti...

The hepatotoxin Cylindrospermopsin, a sulfated-guanidinium alkaloid with substituted dioxypyrimidine (uracil) moiety, was isolated from several cyanobacteria species. Our previous studies on the toxicity of cylindrospermopsin and its derivatives suggested that the uracil moiety is crucial for the toxicity and that such toxicity could partly stem from competitive binding of the toxin to a cataly...

A study of the uridine monophosphate kinase (UMPK) electrophoretic patterns in Venezuelan individuals from the mestizo population of Caracas and from the Warao Indians of the Nabasanuka village in the Delta of the Orinoco River are reported. Among the mestizo population, the frequency of the UMPK1, UMPK2, and UMPK3 alleles was .979, .020, and .001, respectively. A higher frequency of the UMPK3 ...

Bovine pancreatic ribonuclease A (RNase A) has a conserved His ... Asp catalytic dyad in its active site. Structural analyses had indicated that Asp121 forms a hydrogen bond with His119, which serves as an acid during catalysis of RNA cleavage. The enzyme contains three other histidine residues including His12, which is also in the active site. Here, 1H-NMR spectra of wild-type RNase A and the ...

OBJECTIVES To describe our novel modified technique of ultra-mini-percutaneous nephrolithotomy (UMP) using of a novel 6 Fr mininephroscope through an 11-13 Fr metal sheath to perform holmium: YAG laser lithotripsy. METHODS The medical records of 36 patients with moderate-sized (<20 mm) kidney stones treated with UMP from April to July 2012 were retrospectively reviewed. Patients were assessed...