نتایج جستجو برای: uncultivated soil

تعداد نتایج: 181318  

2013
Samuel Jacquiod Laure Franqueville Sébastien Cécillon Timothy M. Vogel Pascal Simonet

Chitin is the second most produced biopolymer on Earth after cellulose. Chitin degrading enzymes are promising but untapped sources for developing novel industrial biocatalysts. Hidden amongst uncultivated micro-organisms, new bacterial enzymes can be discovered and exploited by metagenomic approaches through extensive cloning and screening. Enrichment is also a well-known strategy, as it allow...

2013
Sagar Chhabra Dina Brazil John Morrissey James I Burke Fergal O'Gara David N Dowling

Mineral phosphate solubilization (MPS) microorganisms are important for their provision of orthophosphate anions for plant growth promotion activity in soil. In this study, we applied a functional metagenomic approach to identify this trait directly from the microbiome in barley rhizosphere soil that had not received P fertilizer over a 15-year period. A fosmid system was used to clone the meta...

2018
Dirk Schulze-Makuch Dirk Wagner Samuel P Kounaves Kai Mangelsdorf Kevin G Devine Jean-Pierre de Vera Philippe Schmitt-Kopplin Hans-Peter Grossart Victor Parro Martin Kaupenjohann Albert Galy Beate Schneider Alessandro Airo Jan Frösler Alfonso F Davila Felix L Arens Luis Cáceres Francisco Solís Cornejo Daniel Carrizo Lewis Dartnell Jocelyne DiRuggiero Markus Flury Lars Ganzert Mark O Gessner Peter Grathwohl Lisa Guan Jacob Heinz Matthias Hess Frank Keppler Deborah Maus Christopher P McKay Rainer U Meckenstock Wren Montgomery Elizabeth A Oberlin Alexander J Probst Johan S Sáenz Tobias Sattler Janosch Schirmack Mark A Sephton Michael Schloter Jenny Uhl Bernardita Valenzuela Gisle Vestergaard Lars Wörmer Pedro Zamorano

Traces of life are nearly ubiquitous on Earth. However, a central unresolved question is whether these traces always indicate an active microbial community or whether, in extreme environments, such as hyperarid deserts, they instead reflect just dormant or dead cells. Although microbial biomass and diversity decrease with increasing aridity in the Atacama Desert, we provide multiple lines of ev...

1999
Laurent Marilley Michel Aragno

The rhizosphere of Trifolium repens and Lolium perenne was divided into three fractions: the bulk soil; the soil adhering to the roots; and the washed roots (rhizoplane and endorhizosphere). After isolation and puri®cation of DNA from these fractions, 16S rDNA was ampli®ed by PCR and cloned to obtain a collection of 16S rRNA genes representative of the bacterial communities of these three fract...

Journal: :International Journal of Environmental Research 2022

Abstract The study investigated the emissions of volatile organic compounds (VOCs) from a mining soil amended with sewage sludge and irrigated wastewater or without tomato plants. aim is to find out whether amendment irrigation change VOC changes compared uncultivated soil. Soil plant experiments were done in assembled pots. All pots placed inside closed glass chamber an isolated windowless roo...

Journal: :Proceedings of the National Academy of Sciences 2010

Journal: :Annual review of microbiology 2003
Michael S Rappé Stephen J Giovannoni

Since the delineation of 12 bacterial phyla by comparative phylogenetic analyses of 16S ribosomal RNA in 1987 knowledge of microbial diversity has expanded dramatically owing to the sequencing of ribosomal RNA genes cloned from environmental DNA. Currently, only 26 of the approximately 52 identifiable major lineages, or phyla, within the domain Bacteria have cultivated representatives. Evidence...

Journal: :Land 2022

Changes in land use have several impacts on soil organic carbon (C) and nitrogen (N) cycling, both of which are important for stability fertility. Initially, the study area was barren uncultivated desert land. During late 1960s, introduction a canal arid region converted deserts into cultivated The objectives present were to evaluate effects various systems temporal changes C N pools, usefulnes...

Journal: :Vestnik Nižnevartovskogo gosudarstvennogo universiteta 2021

Studies were conducted to determine such indicators as humus, heavy metals, and enzymatic activity in the soils of uncultivated farmlands Vladimir region located Klyazma river basin. In course field research 2018, soil samples selected at 13 points representing various landscape areas belonging According results research, a positive relationship between enzymes content humus was established. fa...

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