objective(s): we aimed to study the effect of trimetazidine (tmz) on urethral wound repair. materials and methods: a total of 52 male rats were used; 8 groups were formed: 1-week and 3-week control (c1,c3), sham (s1, s3), oral (ot1, ot3), and intraurethral tmz (iut1, iut3) groups. serum and urine total antioxidant capacity (tac), total oxidant capacity (toc), and 8-hydroxy-deoxy-guanosine (8-oh...

This study aimed at detecting and determining the prevalence of genital pathogens among attendees of sexually transmitted infections (STIs) clinic of a tertiary care hospital in Ibadan, Southwestern Nigeria. One hundred consecutive patients (43 males and 57 females) who attended the STI clinic of a tertiary care hospital at Ibadan, with one or more of the complaints as enunciated by WHO in its ...

From April to September 1993, 305 men and 447 women in Hamilton, Canada, consented to the collection of a urethral or cervical swab, respectively, for culture and 20 ml of first-void urine (FVU) for testing by the enzyme immunoassay Chlamydiazyme and by ligase chain reaction (LCR) in the form of a kit from Abbott Laboratories called LCx Chlamydia trachomatis. Evaluation of test performance with...

Trichomonas vaginalis infection in males has been largely uncharacterized. Past reports indicated increased susceptibility to other sexually transmitted infection (STI) agents such as human immunodeficiency virus and Neisseria gonorrhoeae with concurrent T. vaginalis infection. This warrants a more thorough review of male T. vaginalis incidence. A retrospective 3-year investigation of transcrip...

Isolation of Mycoplasma genitalium from clinical specimens remains difficult. We describe a modified culture system based on Vero cells grown in medium 199 with 2% foetal bovine serum (FBS). The culture system was evaluated using early passage M. genitalium strains M6271 and M6311 with growth monitoring by quantitative TaqMan PCR. Eleven endocervical swabs and one male urethral swab positive by...

Background: This study evaluated the performance of the BD ProbeTec Chlamydia trachomatis Q (CTQ) Amplified DNA Assay on the BD Viper System with XTR Technology in a multicenter study. Methods: Specimens were collected at 7 geographically diverse clinical sites from 1538 women and men attending sexually transmitted disease, family planning, and obstetrics and gynecology clinics. There were 1465...

In an attempt to increase Chlamydiazyme (Abbott Laboratories) detection of Chlamydia trachomatis antigen and to establish the reproducibility of positive results, we carried out an investigation into the usefulness of testing duplicate specimens, of more aggressive endocervical specimen collection by using cytobrushes instead of swabs, and of the repeated testing of both specimens from patients...

Background: This study was undertaken to determine co-infection rates of common aetiological agents of urethritis and information regarding sexual contacts of symptomatic males presenting to a family practitioner. Methods: Endo-urethral swab specimens were collected from 253 symptomatic men and tested for N. gonorrhoeae, C. trachomatis by the BD ProbeTec TM ET assay and for T. vaginalis by real...

Urethral or cervical swab of 255 patients attending Skin and Social Hygiene Centre and found positive for gram negative intracellular diplococci on direct microscopy were inoculated on Modified New York City (MNYC) medium and chocolate (heated blood) agar for isolation of neisseria gonorrhea. Growth of N. gonorrhea was obtained in 134 (52.5%) cases. These strains were tested for penicillin susc...

We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and LGV reference strains and did not cross-react with the DNA from 26 strains repr...