Oligosaccharides, also termed sugar oligomers, refer to short-chain polymers of monosaccharide units connected by a and/or b glycosidic bonds. In structure, oligosaccharides represent a class of carbohydrates between polysaccharides and monosaccharides, but the range of degree of polymerization (DP, chain length) spanned by oligosaccharides has not been consistently defined. For example, the Me...

Oligosaccharides are carbohydrates with a low polymerization degree containing between three and fifteen monosaccharide residues covalently linked through glycosidic bonds. related to plant defense responses possess beneficial attributes for human health. Research has focused in wine oligosaccharides only the last decade. In this paper, summary of these works is provided. They include: (i) orig...

Banana’s pseudostem pulp (BPP) is a potential by-product obtained in the mechanical fiber extraction of banana’s pseudostem. Its chemical characterization revealed to have an interesting composition, with high polysaccharides content and low lignin, which makes it particularly relevant for biorefinery’s biochemical platform. Autohydrolysis pretreatment, studied under isothermal (140 °C) non-iso...

Preterm birth still causes significant mortality and morbidity in newborns, despite modern advancements preterm care. The gut microbiota is the most microbial colonization human body plays a role supporting healthy body. infants are susceptible to dysbiosis, which thought contribute many adverse outcomes of prematurity, such as necrotizing enterocolitis (NEC), late-onset sepsis (LOS), developme...

Immunoglobulins (IgG) are soluble serum glycoproteins in which the oligosaccharides play significant roles in the bioactivity and pharmacokinetics. Recombinant immuno-globulins (rIgG) produced in different host cells by recombinant DNA technology are becoming major therapeutic agents to treat life threatening diseases such as cancer. Since glycosylation is cell type specific, rIgGs produced in ...

The structures of the N-linked oligosaccharides of momordin-a, which is a ribosome-inactivating protein from the seeds of Momordica charantia, were analyzed. First, the N-linked oligosaccharides of this glycoprotein were liberated by hydrazinolysis. After N-acetylation, the reducing ends of the oligosaccharides were coupled with 2-aminopyridine and the pyridylamino (PA-) derivatives were purifi...

A method was developed to isolate monoand disaccharide-free oligosaccharides from human and bovine milk using a combination of enzymatic digestion of lactose and solid-phase extraction. In the initial trial, 2.5 g of oligosaccharides were isolated from one liter of human milk. In subsequent trials this was increased to over 5 g of oligosaccharides per liter. Compared to filtration-based extract...

Over forty-five complex free oligosaccharides (of which several are novel) have been isolated and chemically characterized by gas chromatography and high resolution and high mass accuracy matrix-assisted laser desorption/ionization mass spectrometry (MALDI-FTICR MS) in red and white wines, Grignolino and Chardonnay, respectively. Oligosaccharides with a degree of polymerization between 3 and 14...

Heparan sulfate is a sulfated glycan that exhibits essential physiological functions. Interrogation of the specificity of heparan sulfate-mediated activities demands a library of structurally defined oligosaccharides. Chemical synthesis of large heparan sulfate oligosaccharides remains challenging. We report the synthesis of oligosaccharides with different sulfation patterns and sizes from a di...

AIMS Comparison of in vitro fermentation properties of commercial prebiotic oligosaccharides. METHODS AND RESULTS Populations of predominant gut bacterial groups were monitored over 24 h of batch culture through fluorescent in-situ hybridization. Short-chain fatty acid and gas production were also measured. All prebiotics increased the numbers of bifidobacteria and most decreased clostridia. ...