Investigating the optical properties of various chemical compounds using UV–vis spectrophotometers is an essential part education in chemistry. However, commercial are usually treated as “magic black boxes”, where dominant majority elements hidden “under hood”. This often limits understanding mechanisms behind generation spectral curves, which turn may impede ability to understand limitations a...

A new reflectometer-spectrophotometer has been designed and constructed using state-of-the-art technology to enhance optical properties of materials measurements over the ultraviolet, visible, and near-infrared (UV-Vis-NIR) wavelength range (200 nm to 2500 nm). The instrument, Spectral Tri-function Automated Reference Reflectometer (STARR), is capable of measuring specular and diffuse reflectan...

Spectrophotometers are usually built based on one of these two kinds of geometry; d/8 (diffuse/8) and 45/0. Considering that measured or observed colors depend on the illuminating and viewing conditions, the 45/0 spectrophotometer geometry or d/8 geometry affects the results. In addition, the sample characteristics such as gloss or texture can also influence the measuring results. Therefore, th...

This study was carried out to determine the major phospholipids in selected dairy products (Beta Serum, Procream and Phospholipids-Rich Dairy products), evaluate accuracy of developed fat extraction method for liquid samples compare different obtained using HPLC-UVvis 31P-NMR methods. It found that can be used estimate lipid content were still a bit satisfactory. Using method, it sphingomyelin ...