A novel HPLC method for the determination of valproic acid (VPA) in human serum using nonanoic acid as internal standard (I.S.) is described in this article. The eluates were separated with a reverse phase column of the dimensions C18 250 × 4.6 mm internal diameter maintained at a temperature of 48 °C. A mobile phase consisting of acetonitrile and 29mM phosphate buffer (pH 3.5) 49: 51 v/v was u...

The urine and plasma of epileptic patients receiving therapeutic doses of valproic acid (2-propyl- pentanoic acid) was found to contain five doubly unsaturated metabolites of valproic acid, which were identified as their trimethylsilyl derivatives by GC/MS. A series of reference substances was synthesized but only two of them were identical with native metabolites: 2(2-propenyl)-4-pentenoic aci...

Download pose: Histone deacetylase inhibitors (HDACi) are targeted anticancer agents with a well-documenility to act synergistically with cytotoxic agents. We recently showed that the HDACi valproic acid izes osteosarcoma cells to doxorubicin in vitro and in vivo. As there are no published reports on the l utility of HDACi in dogs with spontaneous cancers, we sought to determine a safe and biol...

Valproic acid currently is used as an anticonvulsant medication with a broad spectrum of activity. It usually is used in seizures in children and adults that are not controllable by other anticonvulsants. A documented side effect of valproic acid therapy has been the elevation of the serum ammonia level , with the subsequent development of hyperammonemic encephalopathy. We report the brain magn...

To the Editor: pliance with therapy. Annual rates of vaso-occlusive crisis were calculated as previously described by dividing the number of crises by the number of years of therapy (for example, 2 crises Increased synthesis of fetal hemoglobin may ameliorate the cliniin 6 months Å 4 crises per year). The number of days spent in cal severity of sickle cell disease; both chemotherapeutic and non...

BACKGROUND The clinical use of chromatin-modulating drugs, such as histone deacetylase inhibitors, for the treatment of bone marrow failure and hematopoietic malignancies has increased dramatically over the last few years. Nonetheless, little is currently known concerning their effects on myelopoiesis. DESIGN AND METHODS We utilized an ex vivo differentiation system in which umbilical cord bl...

To the Editor: pliance with therapy. Annual rates of vaso-occlusive crisis were calculated as previously described by dividing the number of crises by the number of years of therapy (for example, 2 crises Increased synthesis of fetal hemoglobin may ameliorate the cliniin 6 months Å 4 crises per year). The number of days spent in cal severity of sickle cell disease; both chemotherapeutic and non...

Valproic acid, a branched short-chain fatty acid, has numerous action mechanisms which turn it into a broad spectrum anticonvulsant drug and make its use possible in some other pathologies such as bipolar disorder. It is extensively metabolized in liver, representing β -oxidation in the mitochondria one of its main metabolic route (40%). Carnitine is responsible for its entry into the mitochond...

BACKGROUND A patient with an early diagnosed epilepsy Valproic acid is one of the most widely used antiepileptic drugs. Hyperammonemic encephalopathy is a rare, but potentially fatal, adverse drug reaction to valproic acid. CASE DESCRIPTION A patient with an early diagnosed epilepsy, treated with valproic acid, experienced an altered mental state after 10 days of treatment. Valproic acid seru...