Branched-chain 2-oxo acids which are formed by transamination of leucine, isoleucine, and valine are metabolized by the peroxisomes from mung bean (Vigna radiata L.) hypocotyls. Acylcoenzyme A (CoA) thio ester intermediates of the pathways were separated by reversed-phase high performance liquid chromatography. Retention time and cochromatography of individual acyl-CoA reference standards were ...

Peroxisomes from mung bean (Vigna radiata L.) hypocotyls catalyze, in the presence of branched-chain 2-oxo fatty acid, CoASH and NAD, the release of CO(2), and the formation of NADH and acyl-CoA. The acyl-CoA contains one carbon atom less than the branched-chain 2-oxo fatty acid and serves as substrate for the peroxisomal acyl-CoA oxidase. CO(2) release, NADH and acyl-CoA formation occur in 1:1...

The effect of cadmium chloride on green gram (Vigna radiata Linn.) was studied using the parameters like carbohydrates, protein, chlorophyll and oxidative enzymes like superoxide dismutase, catalase and peroxidase of leaves inorder to know the possible involvement of cadmium metal in the generation of oxidative stress. Results obtained in this work showed that the application of cadmium decreas...

A procedure was developed for the partial purification of succinate dehydrogenase from mung bean (Vigna radiata L.) hypocotyls and soybean (Glycine max [L] Merr. v. Ransom) cotyledons. The procedure utilized a Triton X-100 extraction followed by ammonium sulfate precipitation. The final fraction was enriched in two polypeptides with approximate molecular weights of 67,000 and 30,000 daltons, ex...

In the present study attempts have been made to characterize urease expression in slow growing Bradyrhizobium strains TAL442 and MO5 which are endosymbionts of green gram (Vigna radiata (L.)Wilczek). It was found that urease activity in vegetative cells of both the strains was inducible unlike their fast growing counterparts. Mode of regulation in TAL442 was governed by presence of ammonia. Ure...

We describe a simple and cost effective method for screening the effects, of stem extract along with biogenic nanoparticles synthesized from Cissus quadrangularis stem extract, on Vigna radiata seed germination and root length. At higher concentrations (200 μl to 300 μl) of stem extract (1 2.5 g ml ) germination was completely inhibited. The inhibition was dose dependent. Synthesized nanopartic...

INTRODUCTION The genus Vigna Fabaceae, formerly Leguminosae, is composed of more than 200 species that are native to the warm regions of both the old world and new world. Vigna is closely related to Phaseolus, which is composed of more than 20 species that are native to warm or tropical regions of the New World. A number of species previously placed in Phaseolus are now placed in Vigna. The gen...

To verify whether aluminium phytotoxicity causes oxidative stress in developing greengram seedlings, the present investigation was carried out. An uniform decrease in root and shoot elongations was marked as the primary signs of aluminium injury. A significant increase in lipid peroxidation measured in terms of TBARS content was noticed which was also correlated with an increase in membrane inj...

Greengram or mungbean (Vigna radiata(L.) Wilczek) is a well-known grain legume in Asian countries. Among the different DNA markers, microsatellite or simple sequence repeats (SSRs) are the markers of choice for various genetic studies due to co-dominant nature, loci specificity and high reproducibility. In the present study, a set of thirty-five microsatellite primer pairs derived from adzukibe...

Cytotoxic properties of plant extracts and drugs being developed for cancer treatment are usually evaluated by a variety of in vivo and in vitro tests carried out in animal or plant based models. In the present study we have evaluated the possibility of using the germinating mung beans (Vigna radiata), for rapid and inexpensive screening of drugs exhibiting cytotoxic properties. Mung beans were...