نتایج جستجو برای: viral vector

تعداد نتایج: 341208  

Journal: :iranian journal of virology 0
sh shahsavandi razi vaccine and serum research institute, p.o. box 31975-148, karaj, iran mm ebrahimi razi vaccine and serum research institute, p.o. box 31975-148, karaj, iran k sadeghi razi vaccine and serum research institute, p.o. box 31975-148, karaj, iran

background and aims: the caspases are unique proteases that mediate the host cell apoptosis during viral infection. in this study, we identified the caspase cleavage motifs of h5n1 and h9n2 influenza viruses isolated during 1998-2012. materials and methods: amino acid sequences of the eleven proteins encoded by the viruses as the caspase substrates downloaded from ncbi. the caspase cleavage mot...

2017
Naomie Tidd Jacob Michelsen Bryan Hilbert Jane C. Quinn

Gene-directed tissue repair offers the clinician, human or veterinary, the chance to enhance cartilage regeneration and repair at a molecular level. Non-viral plasmid vectors have key biosafety advantages over viral vector systems for regenerative therapies due to their episomal integration however, conventional non-viral vectors can suffer from low transfection efficiency. Our objective was to...

2014
Takehiro Ura Kenji Okuda Masaru Shimada

Viral vectors are promising tools for gene therapy and vaccines. Viral vector-based vaccines can enhance immunogenicity without an adjuvant and induce a robust cytotoxic T lymphocyte (CTL) response to eliminate virus-infected cells. During the last several decades, many types of viruses have been developed as vaccine vectors. Each has unique features and parental virus-related risks. In additio...

Objective(s): Human superoxide dismutase 1 (SOD1) is the cytosolic form of this enzyme it detoxifies superoxide anions and attenuates their toxicities and concomitant detrimental effects on the cells. It is believed that the amount of these enzymes present in the oxidative stress-induced diseases is crucial for preventing disease progression. Transfection of rat bone marrow stromal cells (BMSCs...

Journal: :Human molecular genetics 2016
Johannes C M van der Loo J Fraser Wright

Promising results in several clinical studies have emphasized the potential of gene therapy to address important medical needs and initiated a surge of investments in drug development and commercialization. This enthusiasm is driven by positive data in clinical trials including gene replacement for Hemophilia B, X-linked Severe Combined Immunodeficiency, Leber's Congenital Amaurosis Type 2 and ...

Journal: :Vaccine 2015
Robert T Chen Baevin Carbery Lisa Mac Kenneth I Berns Louisa Chapman Richard C Condit Jean-Louis Excler Marc Gurwith Michael Hendry Arifa S Khan Najwa Khuri-Bulos Bettina Klug James S Robertson Stephen J Seligman Rebecca Sheets Anna-Lise Williamson

Recombinant viral vectors provide an effective means for heterologous antigen expression in vivo and thus represent promising platforms for developing novel vaccines against human pathogens from Ebola to tuberculosis. An increasing number of candidate viral vector vaccines are entering human clinical trials. The Brighton Collaboration Viral Vector Vaccines Safety Working Group (V3SWG) was forme...

Journal: :Cold Spring Harbor protocols 2017
J Simon Wiegert Christine E Gee Thomas G Oertner

Transgenes can be introduced into the cells of organotypic slice cultures using different delivery methods, such as biolistic transfection, electroporation, and viral vector-based transduction. These methods produce different patterns of transgene expression. Local injection of recombinant adeno-associated virus (rAAV) produces a small cluster of transgene-expressing neurons around the injectio...

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