In our previous work we described the preparation and characterization of spray dried hydroxyapatite micro granules loaded with 5-fluorouracil (5-FU). These loaded particles are used as a model drug delivery system (DDS). In this study we examined the in vitro response of two cell lines derived from different tissues to 5-FU loaded granules (LG). Both cell lines, either L929 cells of a mouse fi...

I T has been pointed out by Fell & Canti (1934) as a result of their experiments in vitro concerning the early formation of the avian limb skeleton and kneejoint, that the appearance of the articular rudiment is independent of the bloodand nerve-supply as well as of mechanical influences. These authors believe that the formation of articular surfaces occurs in consequence of the differential gr...

We compared culture results to investigate the influence of antimicrobial-loaded cement on sonicate fluid culture positivity for the diagnosis of prosthetic joint infection. Fifty-four subjects were assessed. The sensitivities of sonicate fluid culture were 77.8% (14 of 18) in subjects with an antimicrobial-loaded cemented prosthesis and 58.3% (21 of 36) in subjects with an antimicrobial-free p...

Background: Curcumin as a yellow natural compound extracted from turmeric root is known it as an antibacterial agent. One of the nanoparticles ability is to decrease the defects of usual drug delivery systems. Chitosan is a low toxic, biodegradable, biocompatible and safe polymer which is used in production of nanoparticles. Nanoparticles like chitosan-tripolyphosphate (TPP) are able to increas...

for half a century, the limitations of obtaining cross-combinations in lilies because of the incompatibility and incongruity between different varieties have been known. somatic hybridization is one of the most powerful tools for achieving distant interspecific hybrids. for this purpose, protoplast preparation is a first and important step in efficient system for the regeneration of plants from...

Rat mesenchymal stem cells (rMSCs) represent a small portion of the cells in the stromal compartment of bone marrow and have the potential to differentiate into bone, cartilage, fat, and fibrous tissue. These mesenchymal progenitor cells were maintained as primary isolates and as subcultured cells in separate closed modular incubator chambers purged with either 95% air and 5% CO(2) (20% or cont...

Advances in biomimetic three-dimensional (3D) melanoma models have brought new prospects of drug screening and disease modeling, since their physiological relevancy for recapitulating vivo tumor architectures is more accurate than traditional two-dimensional (2D) cell culture. Gelatin methacryloyl (GelMA) widely used as a tissue-engineered scaffold hydrogel 3D In the present study, an vitro mal...

The goal of this research is to determine the feasibility of loading rifampin into mesoporous silica nanoparticles. Rifampin was selected as a model lipophilic molecule since it is a well-documented and much used anti tuberculosis drug. The mesoporous silica nanoparticles were prepared by using Tetraethyl ortho silicate and cetyltrimethyl ammonium bromide (as surfactant); The prepared nanoparti...

salt stress negatively impacts crops yield throughout the world. nine varieties of potato (solanum tuberosum l.) were screened for salt stress tolerance by measuring in-vitro growth of the aerial plant parts, as well as roots. salt stress was evaluated by adding 25, 50, 75, 100, 125, 150 and 200 mm of nacl to murashige- skoog (ms) medium and compared to ms medium without nacl. plant length and ...

the aim of the present study was to develop an in vitro system for functional investigation of ovarian follicular cells in sterlet, acipenser ruthenus. oocytes for the primary culture were obtained from the ovaries of a 6 years old sterlet 729 g in weight and 47 cm in total length. the oocytes were in advanced vitellogenesis stage (pi >10). a part of the ovary (containing about 300 follicles) w...