نتایج جستجو برای: western blot analysis
تعداد نتایج: 2962057 فیلتر نتایج به سال:
Western Blot analysis. Protein concentration was measured using BCA kit (Thermo Scientific, Rockford, IL, USA) and protein lysates were separated by SDS-PAGE and then blotted onto Hybond-C extra membranes (GE Healthcare, Buckinghamshire, UK) for Western blot analysis. Antigen-antibody reaction was detected using Super Signal West Pico and Femto chemiluminescent substrate kits according to manuf...
The Western blot techniques that were originally established in the late 1970s are still actively utilized today. However, this traditional method of Western blotting has several drawbacks that include low quality resolution, spurious bands, decreased sensitivity, and poor protein integrity. Recent advances have drastically improved numerous aspects of the standard Western blot protocol to prod...
Objective(s): Juglone is isolated from many species of the Juglandaceae family and used as an anti-viral, anti-bacterial, and anti-tumor therapeutic. Here, we evaluated juglone-induced antitumor effect in ovarian cancer SKOV3 cells. Materials and Methods: MTT assay was performed to examine juglone anti-proliferative effect. Cell cycle and apoptosis were studied using flow cytometry in juglone-t...
Background: The purpose of this study was to examine the impact of Insulin (INS) on human sperm function, in light of a recent proteomic analysis indicating that these cells express the INS receptor (INSR). Materials and Methods: We used Western blot and Immunocytochemical analyses. Results: Immunocytochemical analyses confirmed the presence of INSR in human spermatozoa and localized this recep...
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