FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...

Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

the renowned majority of legal theoreticians (uṣūliyyūn) believe that the premises of ḥikmat are three: 1. the possibility of absoluteness and limitation (iṭlāq wa taqyīd); 2. non-provision of evidence (qarīna); and 3. the speaker being in an expressive position. muḥaqqiq khurāsānī, however, has added a fourth premise, i.e., lack of agreed certitude in conversing face to face (takhātub). some l...

Competing theories on the semantics of group pejorative terms (also known as ‘slurs’) comprise both advocates and opponents to Identity Thesis (IT), according which these their neutral counterparts do not differ in semantic value. In opponents' camp, Christopher Hom has offered an argument based substitution slurs that supports his semanticist approach cast doubts all IT-based approaches slurs....

A foundational assumption in economics is that people are rational: they choose optimal plans of action given their predictions about future states of the world. In games of strategy this means that each player's strategy should be optimal given his or her prediction of the opponents' strategies. We demonstrate that there is an inherent tension between rationality and prediction when players ar...

the political, social transformation in 1940’s, are counted as an importance point in recent history of iran. for increasing the process of reconstruction, mohammad reza shah conquest and destroyed the opponents of this program using military forces and savak (intelligence agency). these opponents believe in working under the mashroote laws. after devastating these opponents, new opponents appe...

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. These quantities were obtained with the recombinant baculovirus/Sf9 insect cell-based expression system in large-scale bioreactor cultures with the use of a serum-free and protein-f...