Taking an innovative approach, a vaccination study using five bacterial strains viz. Vibrio campbelli (B60), V. alginolyticus (B73), V. parahaemolyticus-like (B79), V. parahaemolyticus (R8) and V. harveyi (RG203) was conducted in Penaeus monodon against white spot syndrome virus (WSSV) infection, considered as one of the serious pathogens of shrimps. Oral challenge with shrimps infected with WS...

A protein of 110 kDa (termed VP110) from the envelope fraction of White spot syndrome virus (WSSV) was identified by SDS-PAGE and mass spectrometry. The resulting amino acid sequence matched an open reading frame (wsv035) containing an Arg-Gly-Asp (RGD) motif in the WSSV genome database. To validate the mass-spectrometry result, the C-terminal segment of the wsv035 open reading frame was expres...

JAK/STAT pathway plays an important role in invertebrates during virus infection. However the microRNA (miRNA)-mediated regulation of JAK/STAT is not intensively investigated. Viral miRNAs, encoded by virus genome, have emerged as important regulators in the virus-host interactions. In this study, a WSSV (white spot syndrome virus)-encoded miRNA (WSSV-miR-22) was characterized in shrimp during ...

Several oral vaccination studies have been undertaken to evoke a better protection against white spot syndrome virus (WSSV), a major shrimp pathogen. Formalin-inactivated virus and WSSV envelope protein VP28 were suggested as candidate vaccine components, but their uptake mechanism upon oral delivery was not elucidated. In this study the fate of these components and of live WSSV, orally intubat...

three different experiments were conducted to study the effect of dietary astaxanthin on the immune response, resistance to white spot syndrome virus (wssv) and transcription of antioxidant enzyme genes in pacific white shrimp litopenaeus vannamei. each experiment included one triplicate treatment group (fed with 80 mg/kg astaxanthin supplemented diet) and one triplicate control group (fed with...

In this study, we explored the pathogenic effects of white spot syndrome virus (WSSV) and effects of yeast cell wall in the red claw crayfish, Cherax quadricarinatus, by investigating the activity of enzymes related to innate immune function following artificial infection of immunized and non-immunized crayfish. Our results reveal that the activity of four enzymes, phenoloxidase (PO), peroxidas...

In this study, we explored the pathogenic effects of white spot syndrome virus (WSSV) and effects of yeast cell wall in the red claw crayfish, Cherax quadricarinatus, by investigating the activity of enzymes related to innate immune function following artificial infection of immunized and non-immunized crayfish. Our results reveal that the activity of four enzymes, phenoloxidase (PO), peroxidas...

Viruses are ubiquitous and extremely abundant in the marine environment. One of such marine viruses, the white spot syndrome virus (WSSV), has emerged globally as one of the most prevalent, widespread and lethal for shrimp populations. However, at present there is no treatment available to interfere with the unrestrained occurrence and spread of the disease. The recent progress in molecular bio...

Pacific white shrimp (Litopenaeus vannamei) is the most extensively farmed crustacean species in the world. White spot syndrome virus (WSSV) is one of the major pathogens in the cultured shrimp. However, the molecular mechanisms of the host-virus interaction remain largely unknown. In this study, the impact of WSSV infection on host gene expression in the hepatopancreas of L. vannamei was inves...