A method for isolating brush borders from rat kidney homogenates has been developed. Minced tissue, 27 g, is gently homogenized in 20 mu sodium or potassium bicarbonate, pH 8.1. The diluted homogenate, containing large fragments of brush borders, is filtered and then fractionated with the use of combined differential, isopycnic, and ratezonal centrifugations. The purity of the final brush borde...

Meta-analyses of randomized controlled trials (RTC) have confirmed the hypocholesterolaemic effect of oats and oat based fibers. However, the mechanisms by which oats or oat fractions lower cholesterol is not totally clear. Recognizing the important role of the gut microbiome in metabolism and metabolic disease risk, we examined the impact of whole grain oat Granola (WGO) on the human gut micro...

Assessment of transit through the gastrointestinal tract provides useful information regarding gut physiology and patho-physiology. Although several methods are available, each has distinct advantages and limitations. Recently, an ingestible wire-less motility capsule (WMC), similar to capsule video endoscopy, has become available that offers a less-invasive, standardized, radiation-free and of...

Endotoxins in plasma were monitored during treatment in 18 patients hospitalised for acute exacerbation of Crohn's disease: systemic endotoxaemia was found on admission in all but one. The patients were randomly divided into two groups: one receiving treatment with total parenteral nutrition and steroids. To decrease the absorbable endotoxin pool, the other group was additionally treated with w...

We have reported (1) that n-lysine labeled with Cl4 can be incorporated into the proteins of guinea pig liver homogenate under two different conditions. In the one case the enzyme used was the whole homogenate, the optimum pH was near 6.2, there was an obligatory requirement of calcium, and the incorporation was independent of oxygen. This set of conditions is designated below as the “acid calc...

The cashew nut wastes (CNW), more released in the environment by transforming units, are underused based on bioenergy. This study aimed at evaluating biogas production anaerobic codigestion of CNW with various organic feedstocks. Physicochemical parameters and lower heating value (LHV) hull (CH) almond skin (CS) were determined. Batch cultures each waste, supplemented to pig manure (PM), cow du...

1. [75-Se]selenomethionine was administered to four rabbits and after 4 d their kidneys were removed and homogenized. The long-term fate in rats of an oral dose of this kidney homogenate (RK-75-Se) was compared with that of an oral dose of ]75-Se]selenomethionine mixed with unlabelled rabbit kidney homogenate. 2. Urinary adn faecal radioactivities were measured during the 1st week and whole-bod...