OBJECTIVE To evaluate the inhibitory effect of Gnaphalium affine extracts on xanthine oxidase (XO) activity in vitro and to analyze the mechanism of this effect. METHODS In this in vitro study, Kinetic measurements were performed in 4 different inhibitor concentrations and 5 different xanthine concentrations (60, 100, 200, 300, 400 Μmol/L). Dixon and Lineweaver-Burk plot analysis were used to...

The ethanol, methanol and water extracts of Tecoma stans effective against tested for antiinflammatory activity, lipoxygenase, xanthine oxidase and acetycholinesterase inhibitory activity. The methanol, ethanol and water extracts showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation is 86.32, 83.81, 61.39 g/ml and red blood cells membrane stabilization w...

T HE ENZYME, XANTHINE OXIDASE, is thought to play a central role in the mobilization of storage iron from the liver.1 In the presence of xanthine oxidase, xanthine is oxidized to uric acid, the enzyme acting as electron acceptor in the oxidation reaction . ‘ Coupled to the oxidation of uric acid is the reduction of ferric ferritin to the ferrous state. The livers of immature rats contain very l...

Reactive oxygen species (ROS) contribute to neutrophil activation and the development of acute inflammatory processes in which neutrophils play a central role. However, there is only limited information concerning the mechanisms through which extracellular ROS, and particularly cell membrane-impermeable species, such as superoxide, enhance the proinflammatory properties of neutrophils. To addre...

Hyperuricemia is associated with a number of pathological conditions such as gout. Lowering of elevated uric acid level in the blood could be achieved by xanthine oxidase inhibitors and inhibitors of renal urate reabsorption. Some natural compounds isolated from herbs used in traditional Chinese medicine have been previously demonstrated to possess xanthine oxidase inhibitory activities. In the...