development of resistant strains of pathogens to many fungicides as well as fungicide residues in food stuff have been resulted in attemps to develop biological agents as alternative methods to control post-harvest diseases. practical use of microorganisms requires formulated products that meet a number of criteria. in this study different carriers for powder formulation of p.guilliermondi were...

Development of yeast artificial chromosome (Y AC) vectors, molecular cloning of large segments of chromosomal DNA, and their propagation in yeast cells has become feasible. Overlapping Y AC provides a route to the development of physical maps of entire mammalian chromosomes. A rapid method was developed to isolate and sequence termini of Y AC inserts quickly. The Y AC clone is digested wit...

Disseminated candidiasis is an opportunistic infection in immunosuppressed animals by Candida species. The purposes of this study were to determine the predominant candidal forms in different tissues and tissue reactions. Sixteen dogs were selected in this study. The treatment dogs were immunosuppressed by intravenous administration of cyclophosphamide and after 5 days, they were challenged wit...

The main objective of the present study was to develop an efficient and reliable probe to be routinely used for detection of chromosome 13 copy numbers by interphase FISH. To achieve this, a Yeast Artificial Chromosome (YAC) containing sequences specific for human 13q12 (744D11), was cultured and the whole yeast genomic DNA was extracted. The human insert within the isolated DNA was amplified b...

Co-culture of lactic acid bacteria (LAB) and yeast induces specific responses that are not observed in pure culture. Gene expression profiles of Lactobacillus paracasei ATCC 334 co-cultured with Saccharomyces cerevisiae IFO 0216 were analyzed by DNA microarray, and the responses induced by direct contact with the yeast cells were investigated. Coating the LAB cells with recombinant DnaK, which ...

Secretion is a fundamental process in which cells release substances to their external environments. essential for many body functions, including growth, digestion, and cell communication. To be secreted, proteins must pass through various stations inside cells, together form what called the secretory pathway. In this article, I will tell you about pathway stages that go from production up unti...

in this study, the effect of carbon and nitrogen sources, on production of yeast biomass was determined as well as the efficacy of various formulations of candida membranifaciens to decrease blue mold on apple. the media containing sugar cane molasses as carbon sources caused more yeast growth than the media supplemented with permeate powder. yeast extract as nitrogen source produced higher bio...

Radiation-induced polymerization method was applied to the immobolization of yeast cells. The effects of irradiation, cooling and monomer, which are necessary for polymerization, were recovered completely bu subsequent aerobic incubation of yeast cells. The ethanol productive in immobilized yeast cells increased with the increase of aerobic incubation period. The growth of yeast cells in immobi...

The fungivorous amoeba Protostelium aurantium feeds on a wide range of fungal species. image shows amoebae digesting GFP-expressing cells the human pathogenic yeast Candida parapsilosis. For further details, readers are referred to article by Radosa et al. p. e13389 this issue.